| Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins | |
| Wang, Yali1,2; Liu, Xiaoxiang1; Gao, Hong1,2; Zhang, Hong-Mei3; Guo, An-Yuan3; Xu, Jian4; Xu, Xudong1,2 | |
| Corresponding Author | Xu, Xudong(xux@ihb.ac.cn) |
| 2020-03-01 | |
| Source Publication | MOLECULAR BIOLOGY AND EVOLUTION
 |
| ISSN | 0737-4038 |
| Volume | 37Issue:3Pages:849-863 |
| Abstract | It is known that adaptive evolution in permanently cold environments drives cold adaptation in enzymes. However, how the relatively high enzyme activities were achieved in cold environments prior to cold adaptation of enzymes is unclear. Here we report that an Antarctic strain of Chlorella vulgaris, called NJ-7, acquired the capability to grow at near 0 degrees C temperatures and greatly enhanced freezing tolerance after systematic increases in abundance of enzymes/proteins and positive selection of certain genes. Having diverged from the temperate strain UTEX259 of the same species 2.5 (1.1-4.1) to 2.6 (1.0-4.5) Ma, NJ-7 retained the basic mesophilic characteristics and genome structures. Nitrate reductases in the two strains are highly similar in amino acid sequence and optimal temperature, but the NJ-7 one showed significantly higher abundance and activity. Quantitative proteomic analyses indicated that several cryoprotective proteins (LEA), many enzymes involved in carbon metabolism and a large number of other enzymes/proteins, were more abundant in NJ-7 than in UTEX259. Like nitrate reductase, most of these enzymes were not upregulated in response to cold stress. Thus, compensation of low specific activities by increased enzyme abundance appears to be an important strategy for early stage cold adaptation to Antarctica, but such enzymes are mostly not involved in cold acclimation upon transfer from favorable temperatures to near 0 degrees C temperatures. |
| Keyword | Antarctica cold adaptation intraspecies divergence omics enzyme activity Chlorella vulgaris |
| DOI | 10.1093/molbev/msz273 |
| Funding Organization | Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Knowledge Innovation Project of Hubei Province[2017CFA021] ; STS Project of Chinese Academy of Sciences[KFJ-SW-STS-163] ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS[2019FBZ06] |
| WOS Research Area | Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity |
| WOS Subject | Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity |
| WOS ID | WOS:000522244100018 |
| WOS Keyword | CHLORELLA-VULGARIS C-27 ; FREEZING TOLERANCE ; TEMPERATE STRAINS ; IDENTIFICATION ; EMBRYOGENESIS ; ICE ; GENES ; COLD ; EVOLUTION ; MICROORGANISMS |
| Publisher | OXFORD UNIV PRESS |
| Funding Organization | Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/35916 |
| Collection | 藻类生物学及应用研究中心_期刊论文 |
| Corresponding Author | Xu, Xudong |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China 3.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan, Hubei, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Yali,Liu, Xiaoxiang,Gao, Hong,et al. Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins[J]. MOLECULAR BIOLOGY AND EVOLUTION,2020,37(3):849-863. |
| APA | Wang, Yali.,Liu, Xiaoxiang.,Gao, Hong.,Zhang, Hong-Mei.,Guo, An-Yuan.,...&Xu, Xudong.(2020).Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins.MOLECULAR BIOLOGY AND EVOLUTION,37(3),849-863. |
| MLA | Wang, Yali,et al."Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins".MOLECULAR BIOLOGY AND EVOLUTION 37.3(2020):849-863. |
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