IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins
Wang, Yali1,2; Liu, Xiaoxiang1; Gao, Hong1,2; Zhang, Hong-Mei3; Guo, An-Yuan3; Xu, Jian4; Xu, Xudong1,2
Corresponding AuthorXu, Xudong(xux@ihb.ac.cn)
2020-03-01
Source PublicationMOLECULAR BIOLOGY AND EVOLUTION
ISSN0737-4038
Volume37Issue:3Pages:849-863
AbstractIt is known that adaptive evolution in permanently cold environments drives cold adaptation in enzymes. However, how the relatively high enzyme activities were achieved in cold environments prior to cold adaptation of enzymes is unclear. Here we report that an Antarctic strain of Chlorella vulgaris, called NJ-7, acquired the capability to grow at near 0 degrees C temperatures and greatly enhanced freezing tolerance after systematic increases in abundance of enzymes/proteins and positive selection of certain genes. Having diverged from the temperate strain UTEX259 of the same species 2.5 (1.1-4.1) to 2.6 (1.0-4.5) Ma, NJ-7 retained the basic mesophilic characteristics and genome structures. Nitrate reductases in the two strains are highly similar in amino acid sequence and optimal temperature, but the NJ-7 one showed significantly higher abundance and activity. Quantitative proteomic analyses indicated that several cryoprotective proteins (LEA), many enzymes involved in carbon metabolism and a large number of other enzymes/proteins, were more abundant in NJ-7 than in UTEX259. Like nitrate reductase, most of these enzymes were not upregulated in response to cold stress. Thus, compensation of low specific activities by increased enzyme abundance appears to be an important strategy for early stage cold adaptation to Antarctica, but such enzymes are mostly not involved in cold acclimation upon transfer from favorable temperatures to near 0 degrees C temperatures.
KeywordAntarctica cold adaptation intraspecies divergence omics enzyme activity Chlorella vulgaris
DOI10.1093/molbev/msz273
Funding OrganizationKnowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS
Indexed BySCI ; SCI
Language英语
Funding ProjectKnowledge Innovation Project of Hubei Province[2017CFA021] ; STS Project of Chinese Academy of Sciences[KFJ-SW-STS-163] ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS[2019FBZ06]
WOS Research AreaBiochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS IDWOS:000522244100018
WOS KeywordCHLORELLA-VULGARIS C-27 ; FREEZING TOLERANCE ; TEMPERATE STRAINS ; IDENTIFICATION ; EMBRYOGENESIS ; ICE ; GENES ; COLD ; EVOLUTION ; MICROORGANISMS
PublisherOXFORD UNIV PRESS
Funding OrganizationKnowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; Knowledge Innovation Project of Hubei Province ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; STS Project of Chinese Academy of Sciences ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; national special support program Wan-Ren-Ji-Hua of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35916
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, Xudong
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
3.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan, Hubei, Peoples R China
4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yali,Liu, Xiaoxiang,Gao, Hong,et al. Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins[J]. MOLECULAR BIOLOGY AND EVOLUTION,2020,37(3):849-863.
APA Wang, Yali.,Liu, Xiaoxiang.,Gao, Hong.,Zhang, Hong-Mei.,Guo, An-Yuan.,...&Xu, Xudong.(2020).Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins.MOLECULAR BIOLOGY AND EVOLUTION,37(3),849-863.
MLA Wang, Yali,et al."Early Stage Adaptation of a Mesophilic Green Alga to Antarctica: Systematic Increases in Abundance of Enzymes and LEA Proteins".MOLECULAR BIOLOGY AND EVOLUTION 37.3(2020):849-863.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Yali]'s Articles
[Liu, Xiaoxiang]'s Articles
[Gao, Hong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Yali]'s Articles
[Liu, Xiaoxiang]'s Articles
[Gao, Hong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Yali]'s Articles
[Liu, Xiaoxiang]'s Articles
[Gao, Hong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.