IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
De novo transcriptome assembly and sex-biased gene expression in the gonads of Amur catfish (Silurus asotus)
Shen, Fangfang1; Long, Yong2; Li, Fengyang2,3,4; Ge, Guodong2,4; Song, Guili2; Li, Qing2; Qiao, Zhigang1; Cui, Zongbin2
2020-05-01
Source PublicationGENOMICS
ISSN0888-7543
Volume112Issue:3Pages:2603-2614
Abstract

Amur catfish is extensively distributed and cultured in Asian countries. Despite of economic importance, the genomic information of this species remains limited. A reference transcriptome of Amur catfish was assembled and the sex-biased gene expression in the gonads was characterized using RNA-sequencing. The assembled transcriptome of Amur catfish consisted of 74,840 transcripts. The N50, mean length and max length of transcripts are 1970, 1235 and 16,748 bp. Putative sex-specific transcripts were identified and sex-specific expression of the representative genes was verified by RT-PCR. Differential expression analysis identified 5401 ovarybiased and 5618 testis-biased genes. The ovary-biased genes were mainly enriched in pathways such as RNA transport and ribosome biogenesis in eukaryotes. The testis-biased genes were enriched in calcium signaling and cytokine-cytokine receptor interaction, etc. Our data provide a valuable genomic resource for further investigating the genetic basis of sex determination, sex differentiation and sexual dimorphism of catfish.

KeywordAmur catfish Sexual dimorphism de novo assembly Differential gene expression Gonad Sex determination Sex differentiation
DOI10.1016/j.ygeno.2020.01.026
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000519997800054
WOS KeywordANNOTATION ; GENOME ; RNA ; PROGRAM ; TOOL ; DIFFERENTIATION ; IDENTIFICATION ; DIMORPHISM ; ALIGNMENT ; PATHWAYS
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35870
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorCui, Zongbin
Affiliation1.Henan Normal Univ, Fisheries Coll, Xinxiang 453007, Henan, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Shen, Fangfang,Long, Yong,Li, Fengyang,et al. De novo transcriptome assembly and sex-biased gene expression in the gonads of Amur catfish (Silurus asotus)[J]. GENOMICS,2020,112(3):2603-2614.
APA Shen, Fangfang.,Long, Yong.,Li, Fengyang.,Ge, Guodong.,Song, Guili.,...&Cui, Zongbin.(2020).De novo transcriptome assembly and sex-biased gene expression in the gonads of Amur catfish (Silurus asotus).GENOMICS,112(3),2603-2614.
MLA Shen, Fangfang,et al."De novo transcriptome assembly and sex-biased gene expression in the gonads of Amur catfish (Silurus asotus)".GENOMICS 112.3(2020):2603-2614.
Files in This Item:
File Name/Size DocType Version Access License
1-s2.0-S088875431930(3131KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shen, Fangfang]'s Articles
[Long, Yong]'s Articles
[Li, Fengyang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shen, Fangfang]'s Articles
[Long, Yong]'s Articles
[Li, Fengyang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shen, Fangfang]'s Articles
[Long, Yong]'s Articles
[Li, Fengyang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0888754319307608-main.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.