Myxovirus resistance (Mx) gene and its differential expression regulated by three type I and two type II IFNs in mandarin fish, Siniperca chuatsi | |
Li, Li1,2; Chen, Shan Nan2; Laghari, Zubair Ahmed2,4; Huo, Hui Jun1; Hou, Jing2; Huang, Lin2; Li, Nan2; Nie, P.1,2,3 | |
2020-04-01 | |
Source Publication | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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ISSN | 0145-305X |
Volume | 105Issue:1Pages:13 |
Abstract | Interferons (IFNs) can induce the expression of IFN-stimulated genes (ISGs), such as myxovirus resistance (Mx) protein, to inhibit virus replication. In this study, the expression of Mx gene in mandarin fish, and the IFN-sensitive response elements (ISREs) and gamma-interferon activated sites (GASs) in the promoter of Mx gene were analyzed in relation to the stimulation of three distinct type I IFNs, IFNc, IFNd and IFNh, and two type II IFNs, IFN-gamma and IFN-gamma related molecule (IFN-gamma rel). A single Mx gene was found in mandarin fish, and its expression was highly and constitutively observed in all organs/tissues examined. The Mx gene was significantly induced in vivo for 120 h following infectious spleen and kidney necrosis virus (ISKNV) infection. Furthermore, the overexpression and recombinant of IFNh, IFNc, as well as IFN-gamma can significantly induce Mx expression in MFF-1 cells at transcript and protein levels, although all the three type I IFNs and the two type II IFNs can activate the Mx promoter. In addition, ISRE1 which is the proximal one among the three predicted ISREs seems to be the important ISRE for the higher and efficient activation of the Mx promoter. However, the possible interaction between the GASs and type II IFN signalling molecules require further study. |
Keyword | Mx Promoter Transcription IFN Mandarin fish |
DOI | 10.1016/j.dci.2019.103604 |
Indexed By | SCI |
Language | 英语 |
WOS Research Area | Fisheries ; Immunology ; Zoology |
WOS Subject | Fisheries ; Immunology ; Zoology |
WOS ID | WOS:000514254900015 |
WOS Keyword | TROUT ONCORHYNCHUS-MYKISS ; PANCREATIC NECROSIS VIRUS ; INTERFERON TYPE-I ; FUNCTIONAL-CHARACTERIZATION ; ANTIVIRAL ACTIVITY ; SYSTEMATIC IDENTIFICATION ; MOLECULAR-CLONING ; JAPANESE FLOUNDER ; GAMMA-INTERFERON ; PROMOTER |
Publisher | ELSEVIER SCI LTD |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/35845 |
Collection | 其他_期刊论文 |
Corresponding Author | Nie, P. |
Affiliation | 1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 4.Sindh Agr Univ, Dept Vet Parasitol, Tandojam 70060, Sindh, Pakistan |
Recommended Citation GB/T 7714 | Li, Li,Chen, Shan Nan,Laghari, Zubair Ahmed,et al. Myxovirus resistance (Mx) gene and its differential expression regulated by three type I and two type II IFNs in mandarin fish, Siniperca chuatsi[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,105(1):13. |
APA | Li, Li.,Chen, Shan Nan.,Laghari, Zubair Ahmed.,Huo, Hui Jun.,Hou, Jing.,...&Nie, P..(2020).Myxovirus resistance (Mx) gene and its differential expression regulated by three type I and two type II IFNs in mandarin fish, Siniperca chuatsi.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,105(1),13. |
MLA | Li, Li,et al."Myxovirus resistance (Mx) gene and its differential expression regulated by three type I and two type II IFNs in mandarin fish, Siniperca chuatsi".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 105.1(2020):13. |
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