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Myxovirus resistance (Mx) gene and its differential expression regulated by three type I and two type II IFNs in mandarin fish, Siniperca chuatsi
Li, Li1,2; Chen, Shan Nan2; Laghari, Zubair Ahmed2,4; Huo, Hui Jun1; Hou, Jing2; Huang, Lin2; Li, Nan2; Nie, P.1,2,3
2020-04-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume105Issue:1Pages:13
Abstract

Interferons (IFNs) can induce the expression of IFN-stimulated genes (ISGs), such as myxovirus resistance (Mx) protein, to inhibit virus replication. In this study, the expression of Mx gene in mandarin fish, and the IFN-sensitive response elements (ISREs) and gamma-interferon activated sites (GASs) in the promoter of Mx gene were analyzed in relation to the stimulation of three distinct type I IFNs, IFNc, IFNd and IFNh, and two type II IFNs, IFN-gamma and IFN-gamma related molecule (IFN-gamma rel). A single Mx gene was found in mandarin fish, and its expression was highly and constitutively observed in all organs/tissues examined. The Mx gene was significantly induced in vivo for 120 h following infectious spleen and kidney necrosis virus (ISKNV) infection. Furthermore, the overexpression and recombinant of IFNh, IFNc, as well as IFN-gamma can significantly induce Mx expression in MFF-1 cells at transcript and protein levels, although all the three type I IFNs and the two type II IFNs can activate the Mx promoter. In addition, ISRE1 which is the proximal one among the three predicted ISREs seems to be the important ISRE for the higher and efficient activation of the Mx promoter. However, the possible interaction between the GASs and type II IFN signalling molecules require further study.

KeywordMx Promoter Transcription IFN Mandarin fish
DOI10.1016/j.dci.2019.103604
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000514254900015
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; PANCREATIC NECROSIS VIRUS ; INTERFERON TYPE-I ; FUNCTIONAL-CHARACTERIZATION ; ANTIVIRAL ACTIVITY ; SYSTEMATIC IDENTIFICATION ; MOLECULAR-CLONING ; JAPANESE FLOUNDER ; GAMMA-INTERFERON ; PROMOTER
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35845
Collection其他_期刊论文
Corresponding AuthorNie, P.
Affiliation1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
4.Sindh Agr Univ, Dept Vet Parasitol, Tandojam 70060, Sindh, Pakistan
Recommended Citation
GB/T 7714
Li, Li,Chen, Shan Nan,Laghari, Zubair Ahmed,et al. Myxovirus resistance (Mx) gene and its differential expression regulated by three type I and two type II IFNs in mandarin fish, Siniperca chuatsi[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,105(1):13.
APA Li, Li.,Chen, Shan Nan.,Laghari, Zubair Ahmed.,Huo, Hui Jun.,Hou, Jing.,...&Nie, P..(2020).Myxovirus resistance (Mx) gene and its differential expression regulated by three type I and two type II IFNs in mandarin fish, Siniperca chuatsi.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,105(1),13.
MLA Li, Li,et al."Myxovirus resistance (Mx) gene and its differential expression regulated by three type I and two type II IFNs in mandarin fish, Siniperca chuatsi".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 105.1(2020):13.
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