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Disodium 2-oxoglutarate promotes carbon flux into astaxanthin and fatty acid biosynthesis pathways in Haematococcus
Lu, Zhe1,2; Dai, Jingcheng1; Zheng, LingLing1; Teng, Zhuoran1,2; Zhang, Qi1; Qiu, Dongru1; Song, Lirong1,2
2020-03-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume299Issue:1Pages:10
Abstract

Improving carbon availability in astaxanthin production is pivotal in Haematococcus industry. In this study, disodium 2-oxoglutarate (2-OG-2Na) was observed to be a potential carbon regulator to increase the astaxanthin content. To illustrate its efficacy in astaxanthin production, key genes and enzyme were analyzed. Upon 2-OG-2Na treatment, genes ipi, bkt and crtR-b were up regulated, concomitantly, carotenoids and astaxanthin content increased by 15.4% and 14.0% at 120 h, respectively; additionally, Acetyl-CoA carboxylase was activated, consistent with 1.27-fold increase in fatty acids content. PUFAs increased earlier as fatty acids assembly gene fad was up-regulated to 20.56. It was also found that cell division was not compromised. Altogether, it was suggested that increased carbon skeletons were re-directed into the astaxanthin and fatty acids biosynthesis pathway. Furthermore, 2-OG-2Na was applied in ten Haematococcus strains. Of these strains, astaxanthin contents were accelerated with average net increase of 10.48%, exhibiting a scalable paradigm for commercial production.

KeywordHaematococcus pluvialis Disodium 2-oxoglutarate Astaxanthin production Fatty acids biosynthesis Carbon allocation
DOI10.1016/j.biortech.2019.122612
Indexed BySCI
Language英语
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000508117300018
WOS KeywordCAROTENOID BIOSYNTHESIS ; STRESS ; PLUVIALIS ; ACCUMULATION ; MICROALGAE ; BIOMASS ; ALGA ; IDENTIFICATION ; ASSIMILATION ; CULTIVATION
PublisherELSEVIER SCI LTD
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35538
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorSong, Lirong
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Lu, Zhe,Dai, Jingcheng,Zheng, LingLing,et al. Disodium 2-oxoglutarate promotes carbon flux into astaxanthin and fatty acid biosynthesis pathways in Haematococcus[J]. BIORESOURCE TECHNOLOGY,2020,299(1):10.
APA Lu, Zhe.,Dai, Jingcheng.,Zheng, LingLing.,Teng, Zhuoran.,Zhang, Qi.,...&Song, Lirong.(2020).Disodium 2-oxoglutarate promotes carbon flux into astaxanthin and fatty acid biosynthesis pathways in Haematococcus.BIORESOURCE TECHNOLOGY,299(1),10.
MLA Lu, Zhe,et al."Disodium 2-oxoglutarate promotes carbon flux into astaxanthin and fatty acid biosynthesis pathways in Haematococcus".BIORESOURCE TECHNOLOGY 299.1(2020):10.
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