Dynamic Phosphoproteome Profiling of Zebrafish Embryonic Fibroblasts during Cold Acclimation | |
Yan, Junjun1,2; Long, Yong1; Zhou, Tong1,2; Ren, Jing1,2; Li, Qing1; Song, Guili1; Cui, Zongbin1,3 | |
Corresponding Author | Cui, Zongbin(zbcui@ihb.ac.cn) |
2020 | |
Source Publication | PROTEOMICS
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ISSN | 1615-9853 |
Volume | 20Issue:2Pages:11 |
Abstract | Temperature affects almost all aspects of the fish life. To cope with low temperature, fish have evolved the ability of cold acclimation for survival. However, intracellular signaling events underlying cold acclimation in fish remain largely unknown. Here, the formation of cold acclimation in zebrafish embryonic fibroblasts (ZF4) is monitored and the phosphorylation events during the process are investigated through a large-scale quantitative phosphoproteomic approach. In total, 11 474 phosphorylation sites are identified on 4066 proteins and quantified 5772 phosphosites on 2519 proteins. Serine, threonine, and tyrosine (Ser/Thr/Tyr) phosphorylation accounted for 85.5%, 13.3%, and 1.2% of total phosphosites, respectively. Among all phosphosites, 702 phosphosites on 510 proteins show differential regulation during cold acclimation of ZF4 cells. These phosphosites are divided into six clusters according to their dynamic changes during cold exposure. Kinase-substrate prediction reveals that mitogen-activated protein kinase (MAPK) among the kinase groups is predominantly responsible for phosphorylation of these phosphosites. The differentially regulated phosphoproteins are functionally associated with various cellular processes such as regulation of actin cytoskeleton and MAPK signaling pathway. These data enrich the database of protein phosphorylation sites in zebrafish and provide key clues for the elucidation of intracellular signaling networks during cold acclimation of fish. |
Keyword | cold acclimation cold exposure phosphoproteome profiling signal transduction zebrafish embryonic fibroblasts |
DOI | 10.1002/pmic.201900257 |
Funding Organization | Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
Indexed By | SCI ; SCI |
Language | 英语 |
Funding Project | Science Fund for Creative Research Group of National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31872554] ; National Natural Science Foundation of China[31572610] ; National Natural Science Foundation of China[31772836] ; National Natural Science Foundation of China[31401052] |
WOS Research Area | Biochemistry & Molecular Biology |
WOS Subject | Biochemical Research Methods ; Biochemistry & Molecular Biology |
WOS ID | WOS:000506064600001 |
WOS Keyword | DEPENDENT PROTEIN-KINASE ; IN-VIVO ; C-JUN ; MEDIATES COLD ; HIGH-ACCURACY ; HOG PATHWAY ; PHOSPHORYLATION ; TEMPERATURE ; REVEALS ; ACTIVATION |
Publisher | WILEY |
Funding Organization | Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/35514 |
Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
Corresponding Author | Cui, Zongbin |
Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Wuhan 430072, Hubei, Peoples R China |
Recommended Citation GB/T 7714 | Yan, Junjun,Long, Yong,Zhou, Tong,et al. Dynamic Phosphoproteome Profiling of Zebrafish Embryonic Fibroblasts during Cold Acclimation[J]. PROTEOMICS,2020,20(2):11. |
APA | Yan, Junjun.,Long, Yong.,Zhou, Tong.,Ren, Jing.,Li, Qing.,...&Cui, Zongbin.(2020).Dynamic Phosphoproteome Profiling of Zebrafish Embryonic Fibroblasts during Cold Acclimation.PROTEOMICS,20(2),11. |
MLA | Yan, Junjun,et al."Dynamic Phosphoproteome Profiling of Zebrafish Embryonic Fibroblasts during Cold Acclimation".PROTEOMICS 20.2(2020):11. |
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