中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水环境工程研究中心  > 期刊论文
题名: Establishment of a three-step method to evaluate effects of chemicals on development of zebrafish embryo/larvae
作者: Li, Guangyu1; Ye, Huike1, 3; Su, Guanyong4; Han, Zhihua5; Xie, Congxin1; Zhou, Bingsheng3, 6; Letcher, Robert J.; Giesy, John P.4, 7, 8, 9, 10; Yu, Hongxia4; Liu, Chunsheng1, 2
关键词: Chemical screening ; CdCl2 ; TDCIPP ; FTOH ; Developmental toxicity ; Morphology
刊名: CHEMOSPHERE
发表日期: 2017-11-01
DOI: 10.1016/j.chemosphere.2017.07.163
卷: 186, 页:209-217
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
英文摘要: Core endpoints in zebrafish embryos are crucial indicators in screening harmful effects of chemicals. In this study, we established a three-step process to more quantitatively and less-subjective determine effects of chemicals on phenotypes of developing zebrafish embryos. Embryos were exposed to each of two concentrations of the representative chemicals cadmium chloride (CdCl2), tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) or 1H, 1H, 2H, 2H-nonafluoro-1-hexanol (4:2 FTOH) from 0.75 h post-fertilization (hpf) to 96 hpf. After exposure, larvae were imaged by use of a three-step method to describe morphology. Seven points were selected, which resulted in acquisition of 21 lines and 105 angles from images of larvae. Exposure to TDCIPP (0.1 or 0.2 mg/L), CdCl2 (1 or 4 mg/L) or 4:2 FTOH (0.3 or 1 mg/L) significantly changed lengths of some lines and magnitudes of some angles, that resulted in differential scoring of points. Points were then prioritized and directions, distances and trajectories of movement were further described and standard reference values were developed. Movement of the point describing the mouth during embryonic development was found to be a sensitive parameter for assessment of adverse effects of chemicals. The present study provides a new strategy to characterize phenotypes of development of zebrafish embryo/larva following exposure to environmental toxins. (C) 2017 Elsevier Ltd. All rights reserved.
关键词[WOS]: EMBRYO TOXICITY TEST ; FLUCTUATING ASYMMETRY ; TRIS(1,3-DICHLORO-2-PROPYL) PHOSPHATE ; RISK-ASSESSMENT ; DANIO-RERIO ; EXPOSURE ; MODEL ; EMBRYOS/LARVAE ; TOXICOLOGY ; ASSAYS
语种: 英语
项目资助者: National Nature Science Foundation of China (NSFC)(31570510) ; Key Projects in Fok Ying Tung Education Foundation(141078) ; National Science Foundation of Jiangsu Province(BK20130100) ; Canada Research Chair program ; Fundamental Research Funds for the central Universities(2016PY029 ; Huazhong Agricultural University Scientific & Technological Self-Innovation Foundation(2014RC001) ; "High Level Foreign Experts"(GDT20143200016) ; State Administration of Foreign Experts Affairs, P.R. China ; Einstein Professor Program of the Chinese Academy of Sciences ; Discovery Grant from the Natural Science and Engineering Research Council of Canada(326415-07) ; Western Economic Diversification Canada(6578 ; Canada Foundation ; 2662015PY036) ; 6807 ; 000012711)
WOS记录号: WOS:000411846900025
ISSN号: 0045-6535
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/35511
Appears in Collections:水环境工程研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
1-s2.0-S0045653517312134-main.pdf(908KB)----开放获取--View Download

作者单位: 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
2.Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Yuanjiang, Hunan, Peoples R China
3.Carleton Univ, Dept Chem, Ottawa, ON K1S 5B6, Canada
4.Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China
5.MEP, Nanjing Inst Environm Sci, Nanjing 210042, Jiangsu, Peoples R China
6.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
7.Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada
8.Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada
9.Michigan State Univ, Dept Zool, E Lansing, MI 48824 USA
10.Michigan State Univ, Ctr Integrat Toxicol, E Lansing, MI 48824 USA
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Guangyu]'s Articles
[Ye, Huike]'s Articles
[Su, Guanyong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Guangyu]‘s Articles
[Ye, Huike]‘s Articles
[Su, Guanyong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 1-s2.0-S0045653517312134-main.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院水生生物研究所 - Feedback
Powered by CSpace