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Establishment of a three-step method to evaluate effects of chemicals on development of zebrafish embryo/larvae
Li, Guangyu1; Ye, Huike1,3; Su, Guanyong4; Han, Zhihua5; Xie, Congxin1; Zhou, Bingsheng3,6; Letcher, Robert J.; Giesy, John P.4,7,8,9,10; Yu, Hongxia4; Liu, Chunsheng1,2
2017-11-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume186Pages:209-217
AbstractCore endpoints in zebrafish embryos are crucial indicators in screening harmful effects of chemicals. In this study, we established a three-step process to more quantitatively and less-subjective determine effects of chemicals on phenotypes of developing zebrafish embryos. Embryos were exposed to each of two concentrations of the representative chemicals cadmium chloride (CdCl2), tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) or 1H, 1H, 2H, 2H-nonafluoro-1-hexanol (4:2 FTOH) from 0.75 h post-fertilization (hpf) to 96 hpf. After exposure, larvae were imaged by use of a three-step method to describe morphology. Seven points were selected, which resulted in acquisition of 21 lines and 105 angles from images of larvae. Exposure to TDCIPP (0.1 or 0.2 mg/L), CdCl2 (1 or 4 mg/L) or 4:2 FTOH (0.3 or 1 mg/L) significantly changed lengths of some lines and magnitudes of some angles, that resulted in differential scoring of points. Points were then prioritized and directions, distances and trajectories of movement were further described and standard reference values were developed. Movement of the point describing the mouth during embryonic development was found to be a sensitive parameter for assessment of adverse effects of chemicals. The present study provides a new strategy to characterize phenotypes of development of zebrafish embryo/larva following exposure to environmental toxins. (C) 2017 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordChemical Screening Cdcl2 Tdcipp Ftoh Developmental Toxicity Morphology
DOI10.1016/j.chemosphere.2017.07.163
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Nature Science Foundation of China (NSFC)(31570510) ; National Nature Science Foundation of China (NSFC)(31570510) ; Key Projects in Fok Ying Tung Education Foundation(141078) ; Key Projects in Fok Ying Tung Education Foundation(141078) ; National Science Foundation of Jiangsu Province(BK20130100) ; National Science Foundation of Jiangsu Province(BK20130100) ; Canada Research Chair program ; Canada Research Chair program ; Fundamental Research Funds for the central Universities(2016PY029 ; Fundamental Research Funds for the central Universities(2016PY029 ; Huazhong Agricultural University Scientific & Technological Self-Innovation Foundation(2014RC001) ; Huazhong Agricultural University Scientific & Technological Self-Innovation Foundation(2014RC001) ; "High Level Foreign Experts"(GDT20143200016) ; "High Level Foreign Experts"(GDT20143200016) ; State Administration of Foreign Experts Affairs, P.R. China ; State Administration of Foreign Experts Affairs, P.R. China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Discovery Grant from the Natural Science and Engineering Research Council of Canada(326415-07) ; Discovery Grant from the Natural Science and Engineering Research Council of Canada(326415-07) ; Western Economic Diversification Canada(6578 ; Western Economic Diversification Canada(6578 ; Canada Foundation ; Canada Foundation ; 2662015PY036) ; 2662015PY036) ; 6807 ; 6807 ; 000012711) ; 000012711) ; National Nature Science Foundation of China (NSFC)(31570510) ; National Nature Science Foundation of China (NSFC)(31570510) ; Key Projects in Fok Ying Tung Education Foundation(141078) ; Key Projects in Fok Ying Tung Education Foundation(141078) ; National Science Foundation of Jiangsu Province(BK20130100) ; National Science Foundation of Jiangsu Province(BK20130100) ; Canada Research Chair program ; Canada Research Chair program ; Fundamental Research Funds for the central Universities(2016PY029 ; Fundamental Research Funds for the central Universities(2016PY029 ; Huazhong Agricultural University Scientific & Technological Self-Innovation Foundation(2014RC001) ; Huazhong Agricultural University Scientific & Technological Self-Innovation Foundation(2014RC001) ; "High Level Foreign Experts"(GDT20143200016) ; "High Level Foreign Experts"(GDT20143200016) ; State Administration of Foreign Experts Affairs, P.R. China ; State Administration of Foreign Experts Affairs, P.R. China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Discovery Grant from the Natural Science and Engineering Research Council of Canada(326415-07) ; Discovery Grant from the Natural Science and Engineering Research Council of Canada(326415-07) ; Western Economic Diversification Canada(6578 ; Western Economic Diversification Canada(6578 ; Canada Foundation ; Canada Foundation ; 2662015PY036) ; 2662015PY036) ; 6807 ; 6807 ; 000012711) ; 000012711)
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS KeywordEMBRYO TOXICITY TEST ; FLUCTUATING ASYMMETRY ; TRIS(1,3-DICHLORO-2-PROPYL) PHOSPHATE ; RISK-ASSESSMENT ; DANIO-RERIO ; EXPOSURE ; MODEL ; EMBRYOS/LARVAE ; TOXICOLOGY ; ASSAYS
WOS IDWOS:000411846900025
Funding OrganizationNational Nature Science Foundation of China (NSFC)(31570510) ; National Nature Science Foundation of China (NSFC)(31570510) ; Key Projects in Fok Ying Tung Education Foundation(141078) ; Key Projects in Fok Ying Tung Education Foundation(141078) ; National Science Foundation of Jiangsu Province(BK20130100) ; National Science Foundation of Jiangsu Province(BK20130100) ; Canada Research Chair program ; Canada Research Chair program ; Fundamental Research Funds for the central Universities(2016PY029 ; Fundamental Research Funds for the central Universities(2016PY029 ; Huazhong Agricultural University Scientific & Technological Self-Innovation Foundation(2014RC001) ; Huazhong Agricultural University Scientific & Technological Self-Innovation Foundation(2014RC001) ; "High Level Foreign Experts"(GDT20143200016) ; "High Level Foreign Experts"(GDT20143200016) ; State Administration of Foreign Experts Affairs, P.R. China ; State Administration of Foreign Experts Affairs, P.R. China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Discovery Grant from the Natural Science and Engineering Research Council of Canada(326415-07) ; Discovery Grant from the Natural Science and Engineering Research Council of Canada(326415-07) ; Western Economic Diversification Canada(6578 ; Western Economic Diversification Canada(6578 ; Canada Foundation ; Canada Foundation ; 2662015PY036) ; 2662015PY036) ; 6807 ; 6807 ; 000012711) ; 000012711) ; National Nature Science Foundation of China (NSFC)(31570510) ; National Nature Science Foundation of China (NSFC)(31570510) ; Key Projects in Fok Ying Tung Education Foundation(141078) ; Key Projects in Fok Ying Tung Education Foundation(141078) ; National Science Foundation of Jiangsu Province(BK20130100) ; National Science Foundation of Jiangsu Province(BK20130100) ; Canada Research Chair program ; Canada Research Chair program ; Fundamental Research Funds for the central Universities(2016PY029 ; Fundamental Research Funds for the central Universities(2016PY029 ; Huazhong Agricultural University Scientific & Technological Self-Innovation Foundation(2014RC001) ; Huazhong Agricultural University Scientific & Technological Self-Innovation Foundation(2014RC001) ; "High Level Foreign Experts"(GDT20143200016) ; "High Level Foreign Experts"(GDT20143200016) ; State Administration of Foreign Experts Affairs, P.R. China ; State Administration of Foreign Experts Affairs, P.R. China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Discovery Grant from the Natural Science and Engineering Research Council of Canada(326415-07) ; Discovery Grant from the Natural Science and Engineering Research Council of Canada(326415-07) ; Western Economic Diversification Canada(6578 ; Western Economic Diversification Canada(6578 ; Canada Foundation ; Canada Foundation ; 2662015PY036) ; 2662015PY036) ; 6807 ; 6807 ; 000012711) ; 000012711)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35511
Collection水环境工程研究中心
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
2.Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Yuanjiang, Hunan, Peoples R China
3.Carleton Univ, Dept Chem, Ottawa, ON K1S 5B6, Canada
4.Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China
5.MEP, Nanjing Inst Environm Sci, Nanjing 210042, Jiangsu, Peoples R China
6.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
7.Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada
8.Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada
9.Michigan State Univ, Dept Zool, E Lansing, MI 48824 USA
10.Michigan State Univ, Ctr Integrat Toxicol, E Lansing, MI 48824 USA
Recommended Citation
GB/T 7714
Li, Guangyu,Ye, Huike,Su, Guanyong,et al. Establishment of a three-step method to evaluate effects of chemicals on development of zebrafish embryo/larvae[J]. CHEMOSPHERE,2017,186:209-217.
APA Li, Guangyu.,Ye, Huike.,Su, Guanyong.,Han, Zhihua.,Xie, Congxin.,...&Liu, Chunsheng.(2017).Establishment of a three-step method to evaluate effects of chemicals on development of zebrafish embryo/larvae.CHEMOSPHERE,186,209-217.
MLA Li, Guangyu,et al."Establishment of a three-step method to evaluate effects of chemicals on development of zebrafish embryo/larvae".CHEMOSPHERE 186(2017):209-217.
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