| Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus | |
Zhao, Yuanli1,2,3; Liu, Xinhua1,2,3; Xi, Bingwen4; Zhang, Qianqian1,2,3; Li, Aihua1,2,3; Zhang, Jinyong1,2,3
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| Corresponding Author | Zhang, Jinyong(zhangjy@ihb.ac.cn) |
| 2020 | |
| Source Publication | JOURNAL OF INVERTEBRATE PATHOLOGY
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| ISSN | 0022-2011 |
| Volume | 169Pages:13 |
| Abstract | The Myxozoa are endoparasites characterized by a two-host life cycle that typically involves invertebrates and vertebrates as definitive and intermediate hosts, respectively. However, little is known about invertebrate-myxosporean interactions, particularly about patterns of host immune defense. We used RNA-sequencing to identify genes that are possibly involved in the immune responses of the oligochaete Branchiura sowerbyi naturally infected with Myxobolus cilium. De novo assembly of the B. sowerbyi transcriptome yielded 119,031 unigenes, with an average length of 896 bp and an N50 length of 1754 bp. Comparative transcriptome analysis revealed 4059 differentially expressed genes (DEGs) between M. cultus-infected and uninfected B. sowerbyi groups, including 3802 upregulated genes and 257 downregulated genes. Among the B. sowerbyi immune factors implicated in the responses to M. cultus infection, DEGs related to lectins, ubiquitin-mediated proteolysis, phagocytosis, oxidative-antioxidative responses, proteases, and protease inhibitors were upregulated. The expression of some immune-related molecules such as calmodulin, heat shock proteins, antimicrobial peptides, lysenin, and serum amyoid A protein were also significantly upregulated. The expression patterns of 14 immune-related DEGs identified by RNA-seq were validated by quantitative real-time polymerase chain reaction. This study is the first attempt to characterize the B. sowerbyi transcriptome and identify immune-related molecules possibly associated with M. cultus infection. It is also the first report of invertebrate host-myxosporean interactions at the transcriptomic level. Our results will facilitate the elucidation of adaptive evolution mechanisms of myxosporean parasites in the definitive host and the genetic basis for differences in resistance of invertebrate hosts of different genotypes to a myxosporean species. |
| Keyword | Branchiura sowerbyi Myxobolus cultus Innate immune response Transcriptomics Invertebrate-myxosporean interaction |
| DOI | 10.1016/j.jip.2019.107283 |
| Funding Organization | Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Natural Science Foundation of Hubei Province[2018CFA074] ; Goho Life Science International Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund[CX(17)2017] ; State Key Lab of Freshwater Ecology and Biotechnology[2019FBZ02] |
| WOS Research Area | Zoology |
| WOS Subject | Zoology |
| WOS ID | WOS:000506721300006 |
| WOS Keyword | SHRIMP LITOPENAEUS-VANNAMEI ; SCOPHTHALMUS-MAXIMUS ; CYSTEINE-PROTEASES ; CAUSATIVE AGENT ; INNATE IMMUNITY ; LIFE-CYCLE ; GENE ; CEREBRALIS ; MYXOZOA ; INSIGHTS |
| Publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| Funding Organization | Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Natural Science Foundation of Hubei Province ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Goho Life Science International Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; Jiangsu Province Agricultural Science and Technology Independent Innovation Fund ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology ; State Key Lab of Freshwater Ecology and Biotechnology |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/35488 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Zhang, Jinyong |
| Affiliation | 1.Chinese Acad Sci, Minist Agr, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Jiangsu, Peoples R China |
| Recommended Citation GB/T 7714 | Zhao, Yuanli,Liu, Xinhua,Xi, Bingwen,et al. Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2020,169:13. |
| APA | Zhao, Yuanli,Liu, Xinhua,Xi, Bingwen,Zhang, Qianqian,Li, Aihua,&Zhang, Jinyong.(2020).Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus.JOURNAL OF INVERTEBRATE PATHOLOGY,169,13. |
| MLA | Zhao, Yuanli,et al."Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus".JOURNAL OF INVERTEBRATE PATHOLOGY 169(2020):13. |
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