IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9
Sun, Yonghua1; Zhang, Bo2; Luo, Lingfei3; Shi, De-Li4,26,33; Wang, Han5; Cui, Zongbin1; Huang, Honghui3; Cao, Ying6; Shu, Xiaodong7; Zhang, Wenqing8; Zhou, Jianfeng9; Li, Yun9; Du, Jiulin10; Zhao, Qingshun11; Chen, Jun12; Zhong, Hanbing13,27; Zhong, Tao P.14,28; Li, Li3; Xiong, Jing-Wei15; Peng, Jinrong12,29; Xiao, Wuhan1; Zhang, Jian16,30,31; Yao, Jihua17; Yin, Zhan1; Mo, Xianming18; Peng, Gang19; Zhu, Jun20; Chen, Yan21,22; Zhou, Yong22,23; Liu, Dong13; Pan, Weijun21,22,23,24; Zhang, Yiyue8; Ruan, Hua3; Liu, Feng3,24; Zhu, Zuoyan1; Meng, Anming25; Sun, Yonghua; Pan, Luyuan1; Wang, Houpeng1; Xie, Weixun1; He, Mudan1; Ye, Ding1; Li, Kuoyu1; Xiong, Feng1; Liu, Liyue1; Li, Linglu1; Zhang, Yun1; Zhang, Bo; Liu, Da2; Cheng, Zhenchao2; Hu, Yingying2; Wu, Qian2; Luo, Zhou2; Zhang, Yutian2; Wu, Yingdan2; Li, Wenyuan2; Luo, Lingfei; Ma, Jianlong; Mo, Dashuang3; Cai, Pengcheng3; Chen, Jinzi3; Sun, Junhui3; Zhou, Yang3; Wu, Chuan3; Ni, Rui3; Shi, De-Li; Shao, Ming26; Zhang, Yan-Jun26; Feng, Li-Jun26; Cheng, Xiao-Ning26,33; Li, Ji-Tong26; Xing, Yan-Yi26; Zhang, Yan26; Meng, Ya-Ping26; Yu, Bei-Bei26; Wang, Han; Huang, Jian5; Zhang, Shuqing5; Ji, Cheng5; Tan, Yicheng5; Wang, Jingjing5; Zhang, Fanmiao5; Wang, Mingyong5; Huang, Guodong5; Zhong, Zhaomin5; Zhang, Wei5; Cui, Zongbin; Long, Yong1; Song, Guili1; Li, Xiaohui1; Li, Xixi1; Chen, Kai1; Ren, Jing1; Yan, Junjun; Li, Qing1; Huang, Honghui; Ruan, Hua; Zhu, Shicheng3; Chen, Xuejiao3; Pan, Jingjing3; Jiang, Faming3; Chen, Jiehui3; Huang, Chao3; Dong, Guoping3; Yi, Xiaogui3; Cao, Ying; Hu, Ruikun6; Huang, Weilai6; Liu, Jiangfang6; Hong WangXiao6; Shu, Dong7; Xia, Jianhong7; Zhao, Shaoyang7; Wang, Pengtao7; Wu, Xiuhua7; Zhang, Wenqing; Zhang, Yiyue; Xu, Mengchang8; Liu, Wei8; Huang, Zhibing8; Lin, Qing1,8; Wu, Mei8; Zhou, Jianfeng; Sheng, Aibo9; Tang, Peipei9; Gong, Xiaoxia9; Mou, Wei9; Zu, Congcong9; Li, Yun; Lu, Ling9; Liu, Yunzhang9; Rong, Xiaozhi9; Chen, Jianyang9; Du, Jiulin; Bu, Jiwen10; Du, Xufei10; Liu, Tingting10; Gu, Shanye10; Zhao, Qingshun; Dong, Zhangji11; Dong, Xiaohua11; Cao, Shasha11; Yue, Yunyun11; Gu, Chun11; Liu, Meijing11; Chen, Jun; Gong, Hongjian12; Zhong, Hanbing; Yang, Xuanjun13,27; Chen, Jiahao13; Xu, Qiushi13; Zhong, Tao P.; Jin, Daqin28; She, Peilu28; Sun, Jianjian28; Lai, Kaa Seng28; Li, Li; Zhang, Yu3; Shi, Yanyan3; Zhao, Fangying3; Xiong, Jing-Wei; Pang, Meijun15; Gao, Lu15; Lei, Lei15; Peng, Jinrong; Lo, Jane29; Xiao, Wuhan; Cai, Xiaolian1; Ouyang, Gang1; Zhang, Jian; Ma, Weirui32; Miao, Liyun32; Yao, Jihua; Hu, Yu17; Yin, Zhan; Mo, Xianming; Li, Xue18; Peng, Gang; Zhang, Cuizhen19; Wang, Fenghua19; Zhu, Jun; Yang, Ruimeng20; Yuan, Hao20; Chen, Yan; Pan, Yi21,22; Zhou, Yong; Liu, Dong; Zhao, Feng13; Pan, Weijun; Liu, Feng; Ding, Yanyan24; Xue, Yuanyuan24; Wang, Lu24; Zhang, Yifan24; Ma, Dongyuan24; Lu, Xinyan24; Gao, Shuai24; Xia, Jun24; Zhu, Zuoyan; Meng, Anming; Liu, Xingfeng25; Qiu, Juhui25; Gong, Bo25; Chen, Luxi25; Xiong, Cong25; Yao, Likun25; Xing, Cencan25; Wang, Yixia25; Shen, Weimin25; Sun, Jiawei25
Corresponding AuthorLiu, Feng(liuf@ioz.ac.cn) ; Zhu, Zuoyan(zyzhu@ihb.ac.cn) ; Meng, Anming(mengam@mail.tsinghua.edu.cn)
2020
Source PublicationGENOME RESEARCH
ISSN1088-9051
Volume30Issue:1Pages:118-126
AbstractGenome editing by the well-established CRISPR/Cas9 technology has greatly facilitated our understanding of many biological processes. However, a complete whole-genome knockout for any species or model organism has rarely been achieved. Here, we performed a systematic knockout of all the genes (1333) on Chromosome 1 in zebrafish, successfully mutated 1029 genes, and generated 1039 germline-transmissible alleles corresponding to 636 genes. Meanwhile, by high-throughput bioinformatics analysis, we found that sequence features play pivotal roles in effective gRNA targeting at specific genes of interest, while the success rate of gene targeting positively correlates with GC content of the target sites. Moreover, we found that nearly one-fourth of all mutants are related to human diseases, and several representative CRISPR/Cas9-generated mutants are described here. Furthermore, we tried to identify the underlying mechanisms leading to distinct phenotypes between genetic mutants and antisense morpholino-mediated knockdown embryos. Altogether, this work has generated the first chromosome-wide collection of zebrafish genetic mutants by the CRISPR/Cas9 technology, which will serve as a valuable resource for the community, and our bioinformatics analysis also provides some useful guidance to design gene-specific gRNAs for successful gene editing.
DOI10.1101/gr.248559.119
Funding OrganizationMinistry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China
Indexed BySCI ; SCI
Language英语
Funding ProjectMinistry of Science and Technology of China[2018YFA0801000] ; Ministry of Science and Technology of China[2018YFA0800200] ; National Natural Science Foundation of China[31830061] ; National Natural Science Foundation of China[31721005] ; Strategic Priority Research Program of the Chinese Academy of Sciences, China
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000506573000011
WOS KeywordINSERTIONAL MUTAGENESIS ; DRUG DISCOVERY ; WIDE ANALYSIS ; RUNX1 ; CELL ; PROLIFERATION ; MUTATIONS ; PROTEIN ; MODEL ; CAS9
PublisherCOLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
Funding OrganizationMinistry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35482
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorLiu, Feng; Zhu, Zuoyan; Meng, Anming
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Innovat Acad Seed Design, Wuhan 430072, Hubei, Peoples R China
2.Peking Univ, Key Lab Cell Proliferat & Differentiat, Minist Educ, Genome Editing Res Ctr,Coll Life Sci, Beijing 100871, Peoples R China
3.Southwest Univ, Sch Life Sci, Chongqing 400715, Peoples R China
4.Guangdong Med Univ, Zhanjiang 524023, Guangdong, Peoples R China
5.Soochow Univ, Ctr Circadian Clocks, Suzhou 215123, Jiangsu, Peoples R China
6.Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China
7.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Guangdong, Peoples R China
8.South China Univ Technol, Div Cell Dev & Integrat Biol, Sch Med, Guangzhou 510006, Guangdong, Peoples R China
9.Ocean Univ China, Sch Med & Pharm, Qingdao 266100, Shandong, Peoples R China
10.Chinese Acad Sci, Inst Neurosci, State Key Lab Neurosci, Shanghai 200031, Peoples R China
11.Nanjing Univ, Model Anim Res Ctr, Nanjing 210093, Jiangsu, Peoples R China
12.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
13.South Univ Sci & Technol China, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China
14.East China Normal Univ, Inst Biomed Sci, Shanghai 200062, Peoples R China
15.Peking Univ, Inst Mol Med, Coll Life Sci, Beijing 100871, Peoples R China
16.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China
17.Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China
18.Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Sichuan, Peoples R China
19.Fudan Univ, Inst Brain Sci, Shanghai 200433, Peoples R China
20.Shanghai Jiao Tong Univ, Sino French Res Ctr Life Sci & Genom, Rui Jin Hosp, Sch Med, Shanghai 200025, Peoples R China
21.Chinese Acad Sci, Inst Hlth Sci, Shanghai 200025, Peoples R China
22.Shanghai Jiao Tong Univ, Sch Med, Shanghai 200025, Peoples R China
23.Chinese Acad Sci, CAS Key Lab Nutr Metab & Food Safety, Shanghai Inst Nutr & Hlth, Shanghai 200031, Peoples R China
24.Chinese Acad Sci, State Key Lab Membrane Biol, Inst Zool, Beijing 100101, Peoples R China
25.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
26.Shandong Univ, Sch Life Sci, Qingdao 266237, Peoples R China
27.Univ Macau, Inst Chinese Med Sci, Macau, Peoples R China
28.East China Normal Univ, Shanghai Key Lab Regulatory Biol, Inst Biomed Sci, Shanghai 200062, Peoples R China
29.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China
30.State Key Lab Conservat & Utilizat Bioresources, Kunming 650091, Yunnan, Peoples R China
31.Yunnan Univ, Sch Life Sci, Ctr Life Sci, Kunming 650091, Yunnan, Peoples R China
32.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China
33.Guangdong Med Univ, Affiliated Hosp, Zhanjiang 524001, Peoples R China
Recommended Citation
GB/T 7714
Sun, Yonghua,Zhang, Bo,Luo, Lingfei,et al. Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9[J]. GENOME RESEARCH,2020,30(1):118-126.
APA Sun, Yonghua.,Zhang, Bo.,Luo, Lingfei.,Shi, De-Li.,Wang, Han.,...&Sun, Jiawei.(2020).Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9.GENOME RESEARCH,30(1),118-126.
MLA Sun, Yonghua,et al."Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9".GENOME RESEARCH 30.1(2020):118-126.
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