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Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) caused high mortality in farmed rainbow trout (Oncorhynchus mykiss) in China
Xu, Liming1,2; Zhao, Jingzhuang1; Ren, Guangming1; Ying, Dong1; Lin, Jingnan1; Cao, Yongsheng1; Yin, Jiasheng1; Liu, Hongbai1; Lu, Tongyan1; Zhang, Qiya2
2019-10-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume512Issue:1Pages:8
Abstract

The co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) with high mortality was observed in farmed rainbow trout (Oncorhynchus mykiss) in China. Severe extensive necrosis was observed on the major organs of moribund rainbow trout, and tissue fluid from diseased fish was able to cause obvious cytopathic effect in CHSE-214 cells. The co-infection of IHNV and IPNV was detected by RT-PCR and confirmed by an immunofluorescent assay test. The IHNV and IPNV in this co-infection had the same genogroups as the currently prevalent IHNV and IPNV strains in China, and they belonged to genogroup J and genogroup 1, respectively. Distinct from previous studies, the IHNV loads were significantly higher than the IPNV loads in both co-infected tissues and viral cell culture suspension (p < 0.05). Furthermore, the tissue suspensions and cell culture suspensions (cells directly inoculated with pooled tissues suspension from infected fish) were very virulent, causing cumulative mortalities of 94% and 96% in rainbow trout that were challenged at a standard dosage of 100 half tissue culture infective doses (TCID50). Similar high mortality was observed from rainbow trout challenged with purified IHNV, indicating mortality caused by natural co-infected samples may be solely attributable to IHNV, with no apparent impact from the IPNV in the co-infected sample. However, significantly decreased mortality was obtained from the artificial co-infection performed by challenging rainbow trout with a mixture of purified IHNV and purified IPNV at a 1:1 ratio. Notably, viral interference was not detected in this natural co-infection case, even though it could be observed in fish challenged with the purified viruses from the natural infection sample, indicating that IHNV and IPNV co-infection is far more complicated than previously understood. It is urgent to pay more attention to viral interactions to improve the systematic prevention and control of fish diseases.

KeywordCo-infection Infectious hematopoietic necrosis virus Infectious pancreatic necrosis virus Rainbow trout Viral interaction
DOI10.1016/j.aquaculture.2019.734286
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000491992200018
WOS KeywordMOLECULAR EVOLUTION ; SEQUENCE-ANALYSIS ; PHYLOGEOGRAPHY ; GLYCOPROTEIN ; RHABDOVIRUS ; STRAINS
PublisherELSEVIER
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35394
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLu, Tongyan; Zhang, Qiya
Affiliation1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Dept Aquaculture, Lab Fish Dis, Harbin 150070, Heilongjiang, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Xu, Liming,Zhao, Jingzhuang,Ren, Guangming,et al. Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) caused high mortality in farmed rainbow trout (Oncorhynchus mykiss) in China[J]. AQUACULTURE,2019,512(1):8.
APA Xu, Liming.,Zhao, Jingzhuang.,Ren, Guangming.,Ying, Dong.,Lin, Jingnan.,...&Zhang, Qiya.(2019).Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) caused high mortality in farmed rainbow trout (Oncorhynchus mykiss) in China.AQUACULTURE,512(1),8.
MLA Xu, Liming,et al."Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) caused high mortality in farmed rainbow trout (Oncorhynchus mykiss) in China".AQUACULTURE 512.1(2019):8.
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