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High-throughput sequencing reveals the gut and lung prokaryotic community profiles of the Chinese giant salamander (Andrias davidianus)
Wu, Zhenbing1,2; Gatesoupe, Francois-Joel5; Zhang, Qianqian1,3; Wang, Xiehao1; Feng, Yuqing1,2; Wang, Shuyi1,2; Feng, Dongyue4; Li, Aihua1,3
2019-10-01
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume46Issue:5Pages:5143-5154
Abstract

Increasing attention has been attracted to host microbiota, due to their vital impact on host health. Little is known about the microbiota of the Chinese giant salamander (Andrias davidianus), in spite of the high economic and scientific value of this endangered species. This study was designed to characterise and compare the gut and lung prokaryotic communities of the Chinese giant salamander by high-throughput sequencing. Our study showed that the giant salamander had a lung prokaryotic community that clustered separately from its intestinal microbiota. Statistical analysis (LEfSe) revealed that the bacterial populations were dominated by Geobacter, Sulfurimonas, and Dechloromonas from Proteobacteria phylum, and Corynebacterium from Actinobacteria phylum in the lung, while Parabacteroides, Bacteroides, and PW3 from Bacteroidetes phylum, and Oscillospira from Firmicutes phylum were predominant in the intestine. A particularly innovative finding was the fairly high abundance of Archaea, especially methanogenic Euryarchaeota. The gut dominant Archaea were Methanocorpusculum and Thermoplasmata vadinCA11, while Methanosaeta and Methanoculleus were the main Archaea in the lung. PICRUSt analysis revealed differentiated functional profiles between the intestinal miacrobiota and the lung microbiota. Specially, some microbial metabolic functions were significantly more active in the intestinal microbiota, while the functional genes involved in infectious diseases were much richer in the lung microbiota. This study characterized the prokaryotic microbial community profiles in the gut and lung of the Chinese giant salamander, providing foundational support for future study seeking to understand microbiota of the giant salamander and the role of its microbiota on infectious diseases.

KeywordChinese giant salamander (Andrias davidianus) High-throughput sequencing Intestinal tract Lung Prokaryotic community
DOI10.1007/s11033-019-04972-8
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000495590600047
WOS KeywordBACTERIAL COMMUNITIES ; MICROBIOME ; DISEASE ; DIVERSITY ; EVOLUTION ; FISH
PublisherSPRINGER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35372
Collection中科院水生所知识产出(2009年前)
Corresponding AuthorFeng, Dongyue; Li, Aihua
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Hubei, Peoples R China
4.Minist Agr, Natl Fisheries Tech Extens Ctr, Beijing 100125, Peoples R China
5.Ifremer, Ctr Bretagne, Nutr Metab & Aquaculture, INRA, F-29280 Plouzane, France
Recommended Citation
GB/T 7714
Wu, Zhenbing,Gatesoupe, Francois-Joel,Zhang, Qianqian,et al. High-throughput sequencing reveals the gut and lung prokaryotic community profiles of the Chinese giant salamander (Andrias davidianus)[J]. MOLECULAR BIOLOGY REPORTS,2019,46(5):5143-5154.
APA Wu, Zhenbing.,Gatesoupe, Francois-Joel.,Zhang, Qianqian.,Wang, Xiehao.,Feng, Yuqing.,...&Li, Aihua.(2019).High-throughput sequencing reveals the gut and lung prokaryotic community profiles of the Chinese giant salamander (Andrias davidianus).MOLECULAR BIOLOGY REPORTS,46(5),5143-5154.
MLA Wu, Zhenbing,et al."High-throughput sequencing reveals the gut and lung prokaryotic community profiles of the Chinese giant salamander (Andrias davidianus)".MOLECULAR BIOLOGY REPORTS 46.5(2019):5143-5154.
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