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Zebrafish prmt5 arginine methyltransferase is essential for germ cell development
Zhu, Junji1,2; Zhang, Dawei1; Liu, Xing1; Yu, Guangqing1; Cai, Xiaolian1; Xu, Chenxi1; Rong, Fangjing1,2; Ouyang, Gang1; Wang, Jing1; Xiao, Wuhan1,2,3,4,5
2019-10-01
Source PublicationDEVELOPMENT
ISSN0950-1991
Volume146Issue:20Pages:16
Abstract

Protein arginine methyltransferase 5 (Prmt5), a type II arginine methyltransferase, symmetrically dimethylates arginine in nuclear and cytoplasmic proteins. Prmt5 is involved in a variety of cellular processes, including ribosome biogenesis, cellular differentiation, germ cell development and tumorigenesis. However, the mechanisms by which prmt5 influences cellular processes have remained unclear. Here, prmt5 loss in zebrafish led to the expression of an infertile male phenotype due to a reduction in germ cell number, an increase in germ cell apoptosis and the failure of gonads to differentiate into normal testes or ovaries. Moreover, argininemethylation of the germ cell-specific proteins Zili and Vasa, as well as histones H3 (H3R8me2s) and H4 (H4R3me2s), was reduced in the gonads of prmt5-null zebrafish. This resulted in the downregulation of several Piwi pathway proteins, including Zili, and Vasa. In addition, various genes related tomeiosis, gonad development and sexual differentiation were dysregulated in the gonads of prmt5-null zebrafish. Our results revealed a novel mechanism associated with prmt5, i.e. prmt5 apparently controls germ cell development in vertebrates by catalyzing arginine methylation of the germline-specific proteins Zili and Vasa.

KeywordGerm cell Prmt5 Zebrafish Zili Ziwi Vasa
DOI10.1242/dev.179572
Indexed BySCI
Language英语
WOS Research AreaDevelopmental Biology
WOS SubjectDevelopmental Biology
WOS IDWOS:000493947200029
WOS KeywordSM-PROTEIN METHYLTRANSFERASE ; XENOPUS-VASA HOMOLOG ; PIWI PROTEINS ; TUDOR DOMAIN ; RNA-BINDING ; METHYLATION ; GENE ; DIFFERENTIATION ; SPERMATOGENESIS ; EXPRESSION
PublisherCOMPANY BIOLOGISTS LTD
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35366
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorXiao, Wuhan
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China
4.Chinese Acad Sci, Key Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
5.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Junji,Zhang, Dawei,Liu, Xing,et al. Zebrafish prmt5 arginine methyltransferase is essential for germ cell development[J]. DEVELOPMENT,2019,146(20):16.
APA Zhu, Junji.,Zhang, Dawei.,Liu, Xing.,Yu, Guangqing.,Cai, Xiaolian.,...&Xiao, Wuhan.(2019).Zebrafish prmt5 arginine methyltransferase is essential for germ cell development.DEVELOPMENT,146(20),16.
MLA Zhu, Junji,et al."Zebrafish prmt5 arginine methyltransferase is essential for germ cell development".DEVELOPMENT 146.20(2019):16.
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