Zebrafish prmt5 arginine methyltransferase is essential for germ cell development | |
Zhu, Junji1,2; Zhang, Dawei1; Liu, Xing1; Yu, Guangqing1; Cai, Xiaolian1; Xu, Chenxi1; Rong, Fangjing1,2; Ouyang, Gang1; Wang, Jing1; Xiao, Wuhan1,2,3,4,5 | |
2019-10-01 | |
Source Publication | DEVELOPMENT
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ISSN | 0950-1991 |
Volume | 146Issue:20Pages:16 |
Abstract | Protein arginine methyltransferase 5 (Prmt5), a type II arginine methyltransferase, symmetrically dimethylates arginine in nuclear and cytoplasmic proteins. Prmt5 is involved in a variety of cellular processes, including ribosome biogenesis, cellular differentiation, germ cell development and tumorigenesis. However, the mechanisms by which prmt5 influences cellular processes have remained unclear. Here, prmt5 loss in zebrafish led to the expression of an infertile male phenotype due to a reduction in germ cell number, an increase in germ cell apoptosis and the failure of gonads to differentiate into normal testes or ovaries. Moreover, argininemethylation of the germ cell-specific proteins Zili and Vasa, as well as histones H3 (H3R8me2s) and H4 (H4R3me2s), was reduced in the gonads of prmt5-null zebrafish. This resulted in the downregulation of several Piwi pathway proteins, including Zili, and Vasa. In addition, various genes related tomeiosis, gonad development and sexual differentiation were dysregulated in the gonads of prmt5-null zebrafish. Our results revealed a novel mechanism associated with prmt5, i.e. prmt5 apparently controls germ cell development in vertebrates by catalyzing arginine methylation of the germline-specific proteins Zili and Vasa. |
Keyword | Germ cell Prmt5 Zebrafish Zili Ziwi Vasa |
DOI | 10.1242/dev.179572 |
Indexed By | SCI |
Language | 英语 |
WOS Research Area | Developmental Biology |
WOS Subject | Developmental Biology |
WOS ID | WOS:000493947200029 |
WOS Keyword | SM-PROTEIN METHYLTRANSFERASE ; XENOPUS-VASA HOMOLOG ; PIWI PROTEINS ; TUDOR DOMAIN ; RNA-BINDING ; METHYLATION ; GENE ; DIFFERENTIATION ; SPERMATOGENESIS ; EXPRESSION |
Publisher | COMPANY BIOLOGISTS LTD |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/35366 |
Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
Corresponding Author | Xiao, Wuhan |
Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China 4.Chinese Acad Sci, Key Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Hubei, Peoples R China |
Recommended Citation GB/T 7714 | Zhu, Junji,Zhang, Dawei,Liu, Xing,et al. Zebrafish prmt5 arginine methyltransferase is essential for germ cell development[J]. DEVELOPMENT,2019,146(20):16. |
APA | Zhu, Junji.,Zhang, Dawei.,Liu, Xing.,Yu, Guangqing.,Cai, Xiaolian.,...&Xiao, Wuhan.(2019).Zebrafish prmt5 arginine methyltransferase is essential for germ cell development.DEVELOPMENT,146(20),16. |
MLA | Zhu, Junji,et al."Zebrafish prmt5 arginine methyltransferase is essential for germ cell development".DEVELOPMENT 146.20(2019):16. |
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