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Inactivated Mycobacterium vaccine induces innate immunity against M. ulcerans ecovar Liflandii (MuLiflandii ASM001) infection in Dabry's sturgeon (Acipenser dabryanus)
Huang, J.1,2,3; Zhang, S. H.2; Di, J.2; Du, H.2; Wei, Q. W.1,2
2019-10-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume512Issue:1Pages:10
Abstract

Mycobacteriosis, caused by Mycobacterium species, affects nearly 160 fish species worldwide, including several species of sturgeon. It is difficult to detect this disease in the early stage of infection. In addition, there is no effective drug for treatment of the disease in the event of an outbreak. Therefore, an effective vaccine is urgently required. In this study, we evaluated the effects of two candidate vaccines in Dabry's sturgeon, namely, bacillus Calmette-Guerin (BCG) and formalin-killed cells of M. ulcerans ecovar Liflandii (MuLiflandii ASM001; FKC). Dabry's sturgeon injected with sterile phosphate-buffered saline (PBS) was used as a control. The vaccinated individual fish was challenged with MuLiflandii ASM001 to analyze the immunization efficacy of the vaccine. The cumulative mortality of PBS-injected, FKC and BCG vaccinated groups were 8.3%, 3.3%, and 6.7%, respectively. The relative percentage survival of the FKC-vaccinated group reached 60.2%, of the BCG-vaccinated group reached 19.3%. The histopathological assay with Ziehl-Neelsen staining showed that specimens of muscle and middle kidney tissues from the PBS group were positive for acid-fast bacilli. A total of 77,800 unigenes were assembled for transcriptome sequencing, with an average length of 1117 bp. In FKC/PBS and BCG/PBS pairwise comparisons, 1789 and 68 differentially expressed genes (DEGs) were detected, respectively. Twenty-six immune-related genes were differentially expressed among the three different treatment groups. The sequences of the DEGs were annotated and their enrichments were analyzed with Kyoto Encyclopedia of Genes and Genomes and Gene Ontology. FKC/PBS pairwise comparison revealed that the DEGs were significantly enriched in three immune-related pathways: "phagocytosis", "antigen processing and presentation", and "complement and coagulation cascades". These data suggested that this inactivated vaccine induced an innate immune response to MuLiflandii ASM001 infection and may, therefore, be a potential candidate vaccine.

DOI10.1016/j.aquaculture.2019.734312
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000491992200044
WOS KeywordPHAGOCYTOSIS ; FISH ; MARINUM
PublisherELSEVIER
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35363
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorWei, Q. W.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Wuhan 430223, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Huang, J.,Zhang, S. H.,Di, J.,et al. Inactivated Mycobacterium vaccine induces innate immunity against M. ulcerans ecovar Liflandii (MuLiflandii ASM001) infection in Dabry's sturgeon (Acipenser dabryanus)[J]. AQUACULTURE,2019,512(1):10.
APA Huang, J.,Zhang, S. H.,Di, J.,Du, H.,&Wei, Q. W..(2019).Inactivated Mycobacterium vaccine induces innate immunity against M. ulcerans ecovar Liflandii (MuLiflandii ASM001) infection in Dabry's sturgeon (Acipenser dabryanus).AQUACULTURE,512(1),10.
MLA Huang, J.,et al."Inactivated Mycobacterium vaccine induces innate immunity against M. ulcerans ecovar Liflandii (MuLiflandii ASM001) infection in Dabry's sturgeon (Acipenser dabryanus)".AQUACULTURE 512.1(2019):10.
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