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Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus
Yang, Guang1; Yue, Yuewei1; Ren, Silin1; Yang, Mingkun1,2,3; Zhang, Yi1,2; Cao, Xiaohong1,2; Wang, Yinchun1,2; Zhang, Jia3; Ge, Feng3; Wang, Shihua1,2
2019-12-01
Source PublicationENVIRONMENTAL MICROBIOLOGY
ISSN1462-2912
Volume21Issue:12Pages:4792-4807
Abstract

Aspergillus flavus is a pathogenic fungus that produces carcinogenic aflatoxins, posing a great threat to crops, animals and humans. Lysine acetylation is one of the most important reversible post-translational modifications and plays a vital regulatory role in various cellular processes. However, current information on the extent and function of lysine acetylation and aflatoxin biosynthesis in A. flavus is limited. Here, a global acetylome analysis of A. flavus was performed by peptide pre-fractionation, pan-acetylation antibody enrichment and liquid chromatography-mass spectrometry. A total of 1313 high-confidence acetylation sites in 727 acetylated proteins were identified in A. flavus. These acetylation proteins are widely involved in glycolysis/gluconeogenesis, pentose phosphate pathway, citric acid cycle and aflatoxin biosynthesis. AflO (O-methyltransferase), a key enzyme in aflatoxin biosynthesis, was found to be acetylated at K241 and K384. Deletion of aflO not only impaired conidial and sclerotial developments, but also dramatically suppressed aflatoxin production and pathogenicity of A. flavus. Further site-specific mutations showed that lysine acetylation of AflO could also result in defects in development, aflatoxin production and pathogenicity, suggesting that acetylation plays a vital role in the regulation of the enzymatic activity of AflO in A. flavus. Our findings provide evidence for the involvement of lysine acetylation in various biological processes in A. flavus and facilitating in the elucidation of metabolic networks.

DOI10.1111/1462-2920.14825
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000492439500001
WOS KeywordACETYLOME ANALYSIS REVEALS ; PROTEOME-WIDE ANALYSIS ; POSTTRANSLATIONAL MODIFICATIONS ; SECONDARY METABOLISM ; VIRULENCE ; GROWTH ; SUCCINYLATION ; TARGETS ; GENES
PublisherWILEY
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35360
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorWang, Shihua
Affiliation1.Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Fuzhou, Fujian, Peoples R China
2.Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Biopesticide & Chem Biol, Educ Minist, Fuzhou, Fujian, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Yang, Guang,Yue, Yuewei,Ren, Silin,et al. Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus[J]. ENVIRONMENTAL MICROBIOLOGY,2019,21(12):4792-4807.
APA Yang, Guang.,Yue, Yuewei.,Ren, Silin.,Yang, Mingkun.,Zhang, Yi.,...&Wang, Shihua.(2019).Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus.ENVIRONMENTAL MICROBIOLOGY,21(12),4792-4807.
MLA Yang, Guang,et al."Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus".ENVIRONMENTAL MICROBIOLOGY 21.12(2019):4792-4807.
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