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Lysine Propionylation is a Widespread Post-Translational Modification Involved in Regulation of Photosynthesis and Metabolism in Cyanobacteria
Yang, Mingkun1; Huang, Hui1,2; Ge, Feng1
2019-10-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume20Issue:19Pages:21
Abstract

Lysine propionylation is a reversible and widely distributed post-translational modification that is known to play a regulatory role in both eukaryotes and prokaryotes. However, the extent and function of lysine propionylation in photosynthetic organisms remains unclear. Cyanobacteria are the most ancient group of Gram-negative bacteria capable of oxygenic photosynthesis, and are of great importance to global carbon and nitrogen cycles. Here, we carried out a systematic study of lysine propionylaiton in cyanobacteria where we used Synechocystis sp. PCC 6803 (Synechocystis) as a model. Combining high-affinity anti-propionyllysine pan antibodies with high-accuracy mass spectrometry (MS) analysis, we identified 111 unique lysine propionylation sites on 69 proteins in Synechocystis. Further bioinformatic analysis showed that a large fraction of the propionylated proteins were involved in photosynthesis and metabolism. The functional significance of lysine propionylation on the enzymatic activity of fructose-1,6-bisphosphatase (FbpI) was studied by site-directed mutagenesis and biochemical studies. Further functional studies revealed that the propionylation level of subunit II of photosystem I (PsaD) was obviously increased after high light (HL) treatment, suggesting that propionylation may be involved in high light adaption in Synechocystis. Thus, our findings provide novel insights into the range of functions regulated by propionylation and reveal that reversible propionylation is a functional modification with the potential to regulate photosynthesis and carbon metabolism in Synechocystis, as well as in other photosynthetic organisms.

Keywordlysine propionylation post-translational modification Synechocystis sp PCC 6803 cyanobacteria fructose-1 6-bisphosphatase PsaD proteomic analysis
DOI10.3390/ijms20194792
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000494798300133
WOS KeywordACETYLOME ANALYSIS REVEALS ; PHOTOSYSTEM-I COMPLEX ; PSAD SUBUNIT ; HIGH-LIGHT ; MOLECULAR CHARACTERIZATION ; FUNCTIONAL-ANALYSIS ; SYSTEMATIC ANALYSIS ; ELECTRON-TRANSPORT ; PROTEOMIC ANALYSIS ; HYDROGEN-PEROXIDE
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35295
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorGe, Feng
Affiliation1.Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Yang, Mingkun,Huang, Hui,Ge, Feng. Lysine Propionylation is a Widespread Post-Translational Modification Involved in Regulation of Photosynthesis and Metabolism in Cyanobacteria[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(19):21.
APA Yang, Mingkun,Huang, Hui,&Ge, Feng.(2019).Lysine Propionylation is a Widespread Post-Translational Modification Involved in Regulation of Photosynthesis and Metabolism in Cyanobacteria.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(19),21.
MLA Yang, Mingkun,et al."Lysine Propionylation is a Widespread Post-Translational Modification Involved in Regulation of Photosynthesis and Metabolism in Cyanobacteria".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.19(2019):21.
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