Quantitative Proteomics Reveals the Regulatory Networks of Circular RNA CDR1as in Hepatocellular Carcinoma Cells
Yang, Xue1,2; Xiong, Qian1; Wu, Ying1,2; Li, Siting1,2; Ge, Feng1
2017-10-01
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume16Issue:10Pages:3891-3902
AbstractCircular RNAs (circRNAs), a class of widespread endogenous RNAs, play crucial roles in diverse biological processes and are potential biomarkers in diverse human diseases and cancers. Cerebellar-degeneration-related protein 1 antisense RNA (CDRlas), an oncogenic circRNA, is involved in human tumorigenesis and is dysregulated in hepatocellular carcinoma (HCC). However, the molecular mechanisms underlying CDRlas functions in HCC remain unclear. Here we explored the functions of CDR1as and searched for CDRlas-regulated proteins in HCC cells. A quantitative proteomics strategy was employed to globally identify CDRlas-regulated proteins in HCC cells. In total, we identified 330 differentially expressed proteins (DEPs) upon enhanced CDRlas expression in HepG2 cells, indicating that they could be proteins regulated by CDRlas. Bioinformatic analysis revealed that many DEPs were involved in cell proliferation and the cell cycle. Further functional studies of epidermal growth factor receptor (EGFR) found that CDRlas exerts its effects on cell proliferation at least in part through the regulation of EGFR expression. We further confirmed that CDRlas could inhibit the expression of microRNA-7 (miR-7). EGFR is a validated target of miR-7; therefore, CDRlas may exert its function by regulating EGFR expression via targeting miR-7 in HCC cells. Taken together, we revealed novel functions and underlying mechanisms of CDRlas in HCC cells. This study serves as the first proteome-wide analysis of a circRNA-regulated protein in cells and provides a reliable and highly efficient method for globally identifying circRNA-regulated proteins.
SubtypeArticle
KeywordCircular Rnas Cdr1as Hepatocellular Carcinoma Quantitative Proteomics Egfr Mir-7
DOI10.1021/acs.jproteome.7b00519
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31370746) ; National Natural Science Foundation of China(31370746) ; National Key Research and Development Program(2016YFA0501304) ; National Key Research and Development Program(2016YFA0501304) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; National Natural Science Foundation of China(31370746) ; National Natural Science Foundation of China(31370746) ; National Key Research and Development Program(2016YFA0501304) ; National Key Research and Development Program(2016YFA0501304) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202)
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS KeywordGROWTH-FACTOR RECEPTOR ; CANCER CELLS ; LUNG-CANCER ; NONCODING RNAS ; GASTRIC-CANCER ; MIR-7 ; BIOMARKERS ; SYSTEM ; TARGET ; IDENTIFICATION
WOS IDWOS:000412789400036
Funding OrganizationNational Natural Science Foundation of China(31370746) ; National Natural Science Foundation of China(31370746) ; National Key Research and Development Program(2016YFA0501304) ; National Key Research and Development Program(2016YFA0501304) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; National Natural Science Foundation of China(31370746) ; National Natural Science Foundation of China(31370746) ; National Key Research and Development Program(2016YFA0501304) ; National Key Research and Development Program(2016YFA0501304) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202)
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35286
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yang, Xue,Xiong, Qian,Wu, Ying,et al. Quantitative Proteomics Reveals the Regulatory Networks of Circular RNA CDR1as in Hepatocellular Carcinoma Cells[J]. JOURNAL OF PROTEOME RESEARCH,2017,16(10):3891-3902.
APA Yang, Xue,Xiong, Qian,Wu, Ying,Li, Siting,&Ge, Feng.(2017).Quantitative Proteomics Reveals the Regulatory Networks of Circular RNA CDR1as in Hepatocellular Carcinoma Cells.JOURNAL OF PROTEOME RESEARCH,16(10),3891-3902.
MLA Yang, Xue,et al."Quantitative Proteomics Reveals the Regulatory Networks of Circular RNA CDR1as in Hepatocellular Carcinoma Cells".JOURNAL OF PROTEOME RESEARCH 16.10(2017):3891-3902.
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