中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 藻类生物技术和生物能源研发中心  > 期刊论文
题名: Effective control of Poterioochromonas malhamensis in pilot-scale culture of Chlorella sorokiniana GT-1 by maintaining CO2-mediated low culture pH
作者: Ma Mingyang1, 2, 3; Yuan, Danni1, 2, 3; He, Yue1, 2; Park, Minsung1; Gong, Yingchun1, 2; Hu, Qiang1, 2, 4, 5
关键词: Chlorella sorokiniana ; Contamination control ; Poterioochromonas malhamensis ; CO2 ; pH ; Protozoan predator
刊名: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
发表日期: 2017-09-01
DOI: 10.1016/j.algal.2017.06.023
卷: 26, 页:436-444
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Although predators in microalgal culture can often be protozoa reducing biomass productivity and culture stability, there are few effective approaches to control them. This study investigated the effect of culture pH (i.e., 6.0, 6.5, 7.0 and 7.5) maintained by supply of compressed air bubbles containing various concentrations of CO2 on death of the flagellate Poterioochromonas malhamensis and several other protozoa in the culture of the green microalgae Chlorella sorokiniana GT-1. C. sorokiniana GT-1 grew well at pH 6.0 and 6.5 and a sustainable biomass concentration of 1.61 g L-1 was obtained from the cultures maintained at pH 6.5. The cultures maintained at pH 7.0 and 7.5 collapsed on days 7 and 4 of culture, respectively, as a result of contamination by P. malhamensis and to less extent by other protozoa (e.g., ciliates and amoebae). Further experiments revealed that it was the actual dissolved CO2, not the low pH itself, or reduced dissolved oxygen in the culture medium that prevented the occurrence of P. malhamensis. It is speculated that increased CO2 partial pressure in the culture media may enhance diffusion of CO2 into the cytoplasm of P. malhamensis that lowers the intercellular pH, and thus results in cell death. The method developed in this study can be effective in protozoan control in pilot-scale Chlorella culture in an open raceway pond. It is suggested that a low pH maintained temporarily or constantly by supply of CO2 may be a promising approach to control P. malhamensis and alike in microalgal culture.
关键词[WOS]: PRESSURE CARBON-DIOXIDE ; INTRACELLULAR PH ; BIODIESEL PRODUCTION ; INORGANIC CARBON ; MASS-CULTURES ; MICROALGAE ; CONTAMINATION ; INACTIVATION ; GROWTH ; CYANOBACTERIA
语种: 英语
项目资助者: Chinese Academy of Sciences Presidents' International Fellowship Initiative(2016VBA023) ; China Electronics Engineering Design Institute ; State Development & Investment Corporation, China(Y34115-1Z01-0007)
WOS记录号: WOS:000411731800049
ISSN号: 2211-9264
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/35285
Appears in Collections:藻类生物技术和生物能源研发中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.SDIC Microalgae Biotechnol Ctr, China Elect Engn Design Inst, Beijing 100142, Peoples R China
5.Beijing Key Lab Algae Biomass, Beijing 100142, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ma, Mingyang]'s Articles
[Yuan, Danni]'s Articles
[He, Yue]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ma, Mingyang]‘s Articles
[Yuan, Danni]‘s Articles
[He, Yue]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院水生生物研究所 - Feedback
Powered by CSpace