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A transmembrane domain of Andrias davidianus ranavirus 13R is crucial for co-localization to endoplasmic reticulum and viromatrix
Yu, Nai-Tong1,2; Zhang, Qi-Ya1,2
2019-11-01
Source Publication3 BIOTECH
ISSN2190-572X
Volume9Issue:11Pages:10
Abstract

13R, a core gene of Andrias davidianus ranavirus (ADRV), encoded a protein containing a transmembrane domain (TMD) and a restriction endonuclease-like domain. However, the characterization and function of 13R and the protein it encodes remain unclear. In this study, Chinese giant salamander thymus cell (GSTC) was used to investigate the function of 13R. The results showed that the 13R transcripts were detected first at 8 h post-infection (hpi) by RT-PCR and the protein was detected first at 24 hpi by western blot, but the transcription was inhibited by cycloheximide and cytosine arabinofuranoside, indicating that 13R is a viral late gene. Subcellular localization showed that the 13R was co-localized with endoplasmic reticulum (ER) in the cytoplasm, while 13R deleting TMD (13R Delta TM) was distributed in cytoplasm and nucleus. During ADRV infection, 13R was observed first in the cytoplasm and nucleus, and later aggregated into the viromatrix, whereas 13R Delta TM remain dispersed in cytoplasm and nucleus. Western blot analysis suggested that 13R was a viral non-structural protein and its overexpression did not affect the viral titer in GSTC. All these indicated that the TMD of 13R is crucial for the co-localization into the ER and the viromatrix.

KeywordRanavirus Andrias davidianus ranavirus Core gene Transmembrane domain Localization
DOI10.1007/s13205-019-1961-8
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000494451400002
WOS KeywordRANA-GRYLIO VIRUS ; ENVELOPE PROTEIN ; SUBCELLULAR-LOCALIZATION ; IDENTIFICATION ; EXPRESSION ; INFECTION
PublisherSPRINGER HEIDELBERG
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35257
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorYu, Nai-Tong; Zhang, Qi-Ya
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yu, Nai-Tong,Zhang, Qi-Ya. A transmembrane domain of Andrias davidianus ranavirus 13R is crucial for co-localization to endoplasmic reticulum and viromatrix[J]. 3 BIOTECH,2019,9(11):10.
APA Yu, Nai-Tong,&Zhang, Qi-Ya.(2019).A transmembrane domain of Andrias davidianus ranavirus 13R is crucial for co-localization to endoplasmic reticulum and viromatrix.3 BIOTECH,9(11),10.
MLA Yu, Nai-Tong,et al."A transmembrane domain of Andrias davidianus ranavirus 13R is crucial for co-localization to endoplasmic reticulum and viromatrix".3 BIOTECH 9.11(2019):10.
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