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Anti-G250 nanobody-functionalized nanobubbles targeting renal cell carcinoma cells for ultrasound molecular imaging
Yu,Zhiping1; Hu,Ming1; Li,Zhouquan2; Dan Xu,3; Zhu,Lianhua3; Guo,Yanli3; Liu,Qiuli1; Lan,Weihua1; Jiang,Jun1; Wang,Luofu1
2020-02-27
Source PublicationNanotechnology
ISSN0957-4484
Volume31Issue:20Pages:1-14
Abstract

Abstract Traditional imaging examinations have difficulty in identifying benign and malignant changes in renal masses. This difficulty may be solved by ultrasound molecular imaging based on targeted nanobubbles, which could specifically enhance the ultrasound imaging of renal cell carcinomas (RCC) so as to discriminate benign and malignant renal masses. In this study, we aimed to prepare anti-G250 nanobody-functionalized targeted nanobubbles (anti-G250 NTNs) by coupling anti-G250 nanobodies to lipid nanobubbles and to verify their target specificity and binding ability to RCC cells that express G250 antigen and their capacity to enhance ultrasound imaging of RCC xenografts. Anti-G250 nanobodies were coupled to the lipid nanobubbles using the biotin-streptavidin bridge method. The average particle diameter of the prepared anti-G250 NTNs was 446 nm. Immunofluorescence confirmed that anti-G250 nanobodies were uniformly distributed on the surfaces of nanobubbles. In vitro experiments showed that the anti-G250 NTNs specifically bound to G250-positive 786-O cells and HeLa cells with affinities of 88.13%?±?4.37% and 71.8% ±?5.7%, respectively, and that they did not bind to G250-negative ACHN cells. The anti-G250 NTNs could significantly enhance the ultrasound imaging of xenograft tumors arising from 786-O cells and HeLa cells compared with blank nanobubbles, while the enhancement was not significant for xenograft tumors arising from ACHN cells. Immunofluorescence of tumor tissue slices confirmed that the anti-G250 NTNs could enter the tissue space through tumor blood vessels and bind to tumor cells specifically. In conclusion, anti-G250 nanobody-functionalized targeted nanobubbles could specifically bind to G250-positive RCC cells and enhance the ultrasound imaging of G250-positive RCC xenografts. This study has high-potential clinical application value for the diagnosis and differential diagnosis of renal tumors.

Keywordrenal cell carcinoma targeted nanobubble ultrasound molecular imaging nanobody G250 antigen contrast-enhanced ultrasound
DOI10.1088/1361-6528/ab7040
Language英语
WOS IDIOP:0957-4484-31-20-ab7040
PublisherIOP Publishing
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35218
Collection其他_期刊论文
Affiliation1.Department of Urology, Daping Hospital, Army Medical University, Chongqing, People’s Republic of China
2.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People’s Republic of China
3.Department of Ultrasound, Southwest Hospital, Army Medical University, Chongqing, People’s Republic of China
Recommended Citation
GB/T 7714
Yu,Zhiping,Hu,Ming,Li,Zhouquan,et al. Anti-G250 nanobody-functionalized nanobubbles targeting renal cell carcinoma cells for ultrasound molecular imaging[J]. Nanotechnology,2020,31(20):1-14.
APA Yu,Zhiping.,Hu,Ming.,Li,Zhouquan.,Dan Xu,.,Zhu,Lianhua.,...&Wang,Luofu.(2020).Anti-G250 nanobody-functionalized nanobubbles targeting renal cell carcinoma cells for ultrasound molecular imaging.Nanotechnology,31(20),1-14.
MLA Yu,Zhiping,et al."Anti-G250 nanobody-functionalized nanobubbles targeting renal cell carcinoma cells for ultrasound molecular imaging".Nanotechnology 31.20(2020):1-14.
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