IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Steroidogenic acute regulatory protein and luteinizing hormone are required for normal ovarian steroidogenesis and oocyte maturation in zebrafish
Shang, Guohui1; Peng, Xuyan2; Ji, Cheng3,4; Zhai, Gang1; Ruan, Yonglin1,5; Lou, Qiyong1; Jin, Xia1; He, Jiangyan1; Wang, Han3,4; Yin, Zhan1
2019-10-01
Source PublicationBIOLOGY OF REPRODUCTION
ISSN0006-3363
Volume101Issue:4Pages:760-770
Abstract

In recent studies, luteinizing hormone (LH) was reported to play important roles in oocyte maturation. However, the mechanism by which LH signaling, especially regarding the steroidogenesis process, affects oocyte maturation has not been clarified. In this study, zebrafish models with a functional deficiency in luteinizing hormone beta (Lhb) or steroidogenic acute regulatory protein (Star), an enzyme that promotes the transport of cholesterol into the inner mitochondrial membrane for maturation-induced hormone (MIH) production, were generated using transcription activator-like effector nucleases (TALENs). Similar phenotypes of the maturation-arrested oocytes in both female mutants have been observed. The levels of MIH in the oocytes of the female mutants were clearly decreased in both the lhb and star knockout zebrafish. The expression of star was dramatically down-regulated in the lhb mutant follicles and was clearly promoted by forskolin and hCG in vitro. Furthermore, treatment with the MIH precursors, pregnenolone or progesterone, as well as with MIH itself rescued the maturation-arrested oocyte phenotypes in both lhb and star mutants. The plasma levels of other steroids, including testosterone, estradiol, and cortisol, were not affected in the lhb mutants, while the levels of gonad hormones testosterone and estradiol were significantly increased in the star mutants. The cortisol levels were decreased in the star mutants. Collectively, our results confirm that LH plays important roles in the initiation of MIH synthesis from cholesterol and maintains oocyte maturation in zebrafish, as well as provide evidence that Star might act downstream of LH signaling in steroidogenesis. Summary Sentence Steroidogenic acute regulatory protein was a down-stream target of the luteinizing hormone signaling in the regulation of MIH synthesis which maintains oocyte maturation in zebrafish.

Keywordzebrafish lhb star knockout oocyte maturation
DOI10.1093/biolre/ioz132
Indexed BySCI
Language英语
WOS Research AreaReproductive Biology
WOS SubjectReproductive Biology
WOS IDWOS:000509503400011
WOS KeywordTARGETED GENE DISRUPTION ; STAR ; LH ; INSIGHTS ; KNOCKOUT ; CELLS ; DNA
PublisherOXFORD UNIV PRESS INC
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35210
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYin, Zhan
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Zhengzhou Univ, Affiliated Hosp 1, Henan Prov Eye Hosp, Dept Ophthalmol,Henan Int Joint Res Lab Ocular Im, Zhengzhou, Henan, Peoples R China
3.Soochow Univ, Ctr Circadian Clocks, Suzhou, Jiangsu, Peoples R China
4.Soochow Univ, Med Coll, Sch Biol & Basic Med Sci, Dept Genet, Suzhou, Jiangsu, Peoples R China
5.Univ Chinese Acad Sci, Inst Hydrobiol, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Shang, Guohui,Peng, Xuyan,Ji, Cheng,et al. Steroidogenic acute regulatory protein and luteinizing hormone are required for normal ovarian steroidogenesis and oocyte maturation in zebrafish[J]. BIOLOGY OF REPRODUCTION,2019,101(4):760-770.
APA Shang, Guohui.,Peng, Xuyan.,Ji, Cheng.,Zhai, Gang.,Ruan, Yonglin.,...&Yin, Zhan.(2019).Steroidogenic acute regulatory protein and luteinizing hormone are required for normal ovarian steroidogenesis and oocyte maturation in zebrafish.BIOLOGY OF REPRODUCTION,101(4),760-770.
MLA Shang, Guohui,et al."Steroidogenic acute regulatory protein and luteinizing hormone are required for normal ovarian steroidogenesis and oocyte maturation in zebrafish".BIOLOGY OF REPRODUCTION 101.4(2019):760-770.
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