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Characterization and expression of galectin-3 in grass carp (Ctenopharyngodon idella)
Zhu, Denghui1,2; Huang, Rong1; Chu, Pengfei1,2; Chen, Liangming1,2; Li, Yangyu1,2; He, Libo1; Li, Yongming1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1,3
2020-03-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume104Issue:1Pages:9
Abstract

Galectins are members of evolutionary conserved lectin family and play important roles in the innate and adaptive immunity of both vertebrates and invertebrates. Galectin-3 is the only chimera galectin with one Cterminal carbohydrate recognition domain (CRD) connected to the N-terminal end. Here, a galectin-3 (named CiGal3) from grass carp was identified and characterized, which encodes polypeptides 362 amino acids with a predicted molecular mass of 36.45 kDa and theoretical isoelectric point of 4.91. The sugar binding motifs involved in carbohydrate binding activity (H-N-R, V-N and W-E-R) were detected in CRD. In comparison to other species, CiGal3 showed the highest similarity and identity to Cyprinus carpio (95.3% sequence similarity and 92.5% sequence identity). The subcellular localization of CiGal3 was distributed in the cytoplasm and nucleus of transfected cells. The CiGal3 transcripts were ubiquitously expressed in all checked tissues and highly expressed in immune tissues. In addition, the expression of CiGal3 in liver and spleen was induced post grass carp reovirus (GCRV), lipopolysaccharide (LPS), and polyinosinic:polycytidylic acid (poly I:C) challenge. These results suggest that CiGal3 plays a vital role in the immune system.

KeywordGalectin-3 Grass carp reovirus Immune response Subcellular localization mRNA expression
DOI10.1016/j.dci.2019.103567
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000509614700025
WOS KeywordPATTERN-RECOGNITION RECEPTORS ; SHRIMP LITOPENAEUS-VANNAMEI ; GALACTOSIDE-BINDING PROTEIN ; TANDEM-REPEAT GALECTIN-9 ; C-TYPE LECTIN ; MOLECULAR-CLONING ; IMMUNE RECOGNITION ; CHANNEL CATFISH ; GENE ; FAMILY
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35202
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHe, Libo; Wang, Yaping
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Denghui,Huang, Rong,Chu, Pengfei,et al. Characterization and expression of galectin-3 in grass carp (Ctenopharyngodon idella)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,104(1):9.
APA Zhu, Denghui.,Huang, Rong.,Chu, Pengfei.,Chen, Liangming.,Li, Yangyu.,...&Wang, Yaping.(2020).Characterization and expression of galectin-3 in grass carp (Ctenopharyngodon idella).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,104(1),9.
MLA Zhu, Denghui,et al."Characterization and expression of galectin-3 in grass carp (Ctenopharyngodon idella)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 104.1(2020):9.
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