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Human inflammatory dendritic cells in malignant pleural effusions induce Th1 cell differentiation
Gu, Fei-fei1; Wu, Jing-jing1; Liu, Yang-yang1; Hu, Yue1; Liang, Jin-yan1; Zhang, Kai1; Li, Ming2; Wang, Yan3; Zhang, Yong-an4; Liu, Li1
2020-02-12
Source PublicationCANCER IMMUNOLOGY IMMUNOTHERAPY
ISSN0340-7004
Issue1Pages:10
Abstract

Dendritic cells are crucial for the initiation and regulation of immune responses against cancer and pathogens. DCs are heterogeneous and highly specialized antigen-presenting cells. Human DCs comprise several subsets with different phenotypes and functional properties. In the steady state, human DC subsets have been well studied. However, the components of DC subsets and their immune functions during the inflamed setting are poorly understood. We identified and characterized DC subsets in the malignant pleural effusions of NSCLC patients. We analyzed the capacity of these DC subsets to induce T-cell differentiation. We observed the presence of inflammatory DCs (infDCs) and macrophages in the malignant pleural effusions of NSCLC patients, as identified by the CD11C(+)HLA-DR(+)CD16(-)BDCA1(+) and CD11C(+)HLA-DR(+)CD16(+)BDCA1(-) phenotypes, respectively. InfDCs represented approximately 1% of the total light-density cells in the pleural effusion and were characterized by the expression of CD206, CD14, CD11b, and CD1 alpha, which were absent on blood DCs. InfDCs also expressed CD80, although at a low level. As infDCs did not express CD40, CD83 and CD275, they remained functionally immature. We found that TLR agonists promoted the maturation of infDCs. Compared with macrophages, infDCs had a weaker capacity to phagocytose necrotic tumor cell lysates. However, only infDCs induced autologous memory CD4(+) T-cell differentiation into Th1 cells. For the first time, we found that infDCs were present in the malignant pleural effusions of NSCLC patients. We conclude that infDCs represent a distinct human DC subset and induce Th1 cell differentiation in the presence of TLR agonists.

KeywordInflammatory DC NSCLC Pleural effusion DC T cell
DOI10.1007/s00262-020-02510-1
Indexed BySCI
Language英语
WOS Research AreaOncology ; Immunology
WOS SubjectOncology ; Immunology
WOS IDWOS:000513043500002
WOS KeywordPERIPHERAL LYMPHOID ORGANS ; CANCER STATISTICS ; STEADY-STATE ; LUNG-CANCER ; IDENTIFICATION ; MICE ; IMMUNITY ; SUBSETS
PublisherSPRINGER
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35187
Collection其他_期刊论文
Corresponding AuthorLiu, Li
Affiliation1.Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Canc Ctr, 1277 Jiefang Ave, Wuhan 430022, Peoples R China
2.Wuhan Pulm Hosp, Med Oncol, 28 Baofeng Rd, Wuhan 430030, Peoples R China
3.Chinese Acad Sci, Anal & Testing Ctr, Inst Hydrobiol, 7 East Lake South Rd, Wuhan 430072, Peoples R China
4.Huazhong Agr Univ, Coll Fisheries, 1 Shizishan St, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Gu, Fei-fei,Wu, Jing-jing,Liu, Yang-yang,et al. Human inflammatory dendritic cells in malignant pleural effusions induce Th1 cell differentiation[J]. CANCER IMMUNOLOGY IMMUNOTHERAPY,2020(1):10.
APA Gu, Fei-fei.,Wu, Jing-jing.,Liu, Yang-yang.,Hu, Yue.,Liang, Jin-yan.,...&Liu, Li.(2020).Human inflammatory dendritic cells in malignant pleural effusions induce Th1 cell differentiation.CANCER IMMUNOLOGY IMMUNOTHERAPY(1),10.
MLA Gu, Fei-fei,et al."Human inflammatory dendritic cells in malignant pleural effusions induce Th1 cell differentiation".CANCER IMMUNOLOGY IMMUNOTHERAPY .1(2020):10.
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