IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Timing of gonadal development and dimorphic expression of sex-related genes in gonads during early sex differentiation in the Yellow River carp
Jiang, Mouyan1,3; Jia, Shaoting1,3; Chen, Ji1; Chen, Kuangxin1,3; Ma, Wenge1,3; Wu, Xingxing1,3; Luo, Hongrui1,3; Li, Yongming1; Zhu, Zuoyan1; Hu, Wei1,2,3
2020-03-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume518Issue:1Pages:11
Abstract

The common carp, a gonochoristic teleost fish with an XX/XY sex-determining system, provides an excellent model for studying cyprinidae gonadal sex differentiation. The present study aims at investigating the detailed process of early gonadal sex differentiation of common carp by combining the expression patterns of sex related genes (cyp19a1a, foxl2, amh, dmrtl1 sox9b, sycp3, dmc1) with gonadal histological changes using mono-sex female and male Yellow River carp. H&E staining, immunohistochemistry and immunofluorescence of gonads indicated that the ovaries and testis of common carp were both directly differentiated from undifferentiated gonads. Oogonia and spermatogonia were first observed at 40 dph (days post hatching) and 70 dph in female and male gonads, respectively. Female related genes cyp19a1a and foxl2 and male related genes amh, dmrt1 and sox9b increased significantly in 30-40 dph female, and male gonads, respectively. These suggest that the critical timing of sex determination of common carp takes place between 30 and 40 dph or possibly earlier. Meiotic marker gene dmc1 and sycp3 increased expression significantly in 55 dph female gonads while sycp3 increased expression significantly in 100 dph male gonads. Oocytes in meiosis and spermatocytes were first observed in 55 dph female and 100 dph male gonads, respectively. These indicate that the initiation time of gonadal sex differentiation is 50-55 dph in female common carp and 90-100 dph in male common carp. To our knowledge, this is the first study investigating the detailed process of early gonadal sex differentiation of common carp by combining the expression patterns of sex related genes with gonadal histological changes. We have identified the critical window of sex determination and the initiation times of oogenesis and spermatogenesis in the Yellow River carp. These results provide researchers with valuable information to possibly reveal the mechanisms of sex determination and differentiation of common carp.

KeywordYellow River carp All-males All-females Window of sex determination Gonadal sex differentiation
DOI10.1016/j.aquaculture.2019.734825
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000510424900040
WOS KeywordFEMALE COMMON CARP ; CYPRINUS-CARPIO ; Y-CHROMOSOME ; GENOME SEQUENCE ; RAINBOW-TROUT ; VASA RNA ; MEDAKA ; FISH ; SPERMATOGENESIS ; CLONING
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35178
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHu, Wei
Affiliation1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Mouyan,Jia, Shaoting,Chen, Ji,et al. Timing of gonadal development and dimorphic expression of sex-related genes in gonads during early sex differentiation in the Yellow River carp[J]. AQUACULTURE,2020,518(1):11.
APA Jiang, Mouyan.,Jia, Shaoting.,Chen, Ji.,Chen, Kuangxin.,Ma, Wenge.,...&Hu, Wei.(2020).Timing of gonadal development and dimorphic expression of sex-related genes in gonads during early sex differentiation in the Yellow River carp.AQUACULTURE,518(1),11.
MLA Jiang, Mouyan,et al."Timing of gonadal development and dimorphic expression of sex-related genes in gonads during early sex differentiation in the Yellow River carp".AQUACULTURE 518.1(2020):11.
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