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Gnrh3 Regulates PGC Proliferation and Sex Differentiation in Developing Zebrafish
Feng, Ke1; Cui, Xuefan1,2; Song, Yanlong1; Tao, Binbin1; Chen, Ji1; Wang, Jing4; Liu, Shaojun4; Sun, Yonghua1; Zhu, Zuoyan1; Trudeau, Vance L.3; Hu, Wei1,2
Source PublicationENDOCRINOLOGY

Gonadotropin-releasing hormone (Gnrh) plays important roles in reproduction by stimulating luteinizing hormone release, and subsequently ovulation and sperm release, ultimately controlling reproduction in many species. Here we report on a new role for this decapeptide. Surprisingly, Gnrh3-null zebrafish generated by CRISPR/Cas9 exhibited a male-biased sex ratio. After the dome stage, the number of primordial germ cells (PGCs) in gnrh3(-/-) fish was lower than that in wildtype, an effect that was partially rescued by gnrh3 overexpression. A terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) analysis revealed no detectable apoptosis of PGCs in gnrh3(-/-) embryos. Proliferating PGCs could be detected in wild-type embryos, while there was no detectable signal in gnrh3(-/-) embryos. Compared with wild type, the phosphorylation of AKT was not significantly different in gnrh3(-/-) embryos, but the phosphorylation of ERK1/2 decreased significantly. Treatment with a Gnrh analog (Alarelin) induced ERK1/2 phosphorylation and increased PGC numbers in both wild-type and gnrh3(-/-) embryos, and this was blocked by the MEK inhibitor PD0325901. The relative expression of sox9a, amh, and cyp11b were significantly upregulated, while cyp19a1a was significantly downregulated at 18 days post-fertilization in gnrh3(-/-) zebrafish. Taken together, these results indicate that Gnrh3 plays an important role in early sex differentiation by regulating the proliferation of PGCs through a MAPK-dependent path.

KeywordGnrh3 PGC proliferation reproduction sex differentiation gene knockout zebrafish
Indexed BySCI
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000518155500008
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Document Type期刊论文
Corresponding AuthorHu, Wei
Affiliation1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada
4.Hunan Normal Univ, State Key Lab Dev Biol Freshwater Fish, Changsha 410081, Peoples R China
Recommended Citation
GB/T 7714
Feng, Ke,Cui, Xuefan,Song, Yanlong,et al. Gnrh3 Regulates PGC Proliferation and Sex Differentiation in Developing Zebrafish[J]. ENDOCRINOLOGY,2020,161(1):13.
APA Feng, Ke.,Cui, Xuefan.,Song, Yanlong.,Tao, Binbin.,Chen, Ji.,...&Hu, Wei.(2020).Gnrh3 Regulates PGC Proliferation and Sex Differentiation in Developing Zebrafish.ENDOCRINOLOGY,161(1),13.
MLA Feng, Ke,et al."Gnrh3 Regulates PGC Proliferation and Sex Differentiation in Developing Zebrafish".ENDOCRINOLOGY 161.1(2020):13.
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