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Identification of Intracellular Bacteria in the Ciliate Balantidium ctenopharyngodoni (Ciliophora, Litostomatea)
Zhao, Weishan1,2,3; Li, Ming1,2; Xiong, Fan1,2; Zhang, Dong1,2,3; Wu, Shangong1,2; Zou, Hong1,2; Li, Wenxiang1,2; Wang, Guitang1,2
2020-03-01
Source PublicationJOURNAL OF EUKARYOTIC MICROBIOLOGY
ISSN1066-5234
Issue1Pages:10
Abstract

The ciliate Balantidium ctenopharyngodoni is the most prominent protist in the guts of grass carp, where it mainly inhabits the creamy luminal contents of the hindgut. Ciliates are generally colonized by microorganisms via phagotrophic feeding. In order to study the intracellular bacteria in this ciliate, we have successfully established it in in vitro culture. Herein, we investigated and compared the bacterial community structures of cultured and freshly collected B. ctenopharyngodoni. The results showed that these two groups exhibited different bacterial communities. The most abundant bacterial family in freshly collected samples was Enterobacteriaceae, while in cultured samples it was Fusobacteriaceae. In addition, a key intracellular bacterium, Cetobacterium somerae, was identified in the cytoplasm of cultured ciliates using fluorescence in situ hybridization (FISH). This study shows that ciliates can retain the intracellular bacteria acquired in the natural habitat for quite a long time, but the bacterial community structure of ciliates eventually changes after a long period of cultivation.

KeywordBalantidium ctenopharyngodoni Cetobacterium somerae endosymbionts FISH intracellular bacteria
DOI10.1111/jeu.12791
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000517137000001
WOS Keyword16S RIBOSOMAL-RNA ; IN-SITU ; SP-NOV ; POLYNUCLEOBACTER-NECESSARIUS ; METHANOGENIC BACTERIA ; ENDONUCLEAR SYMBIONTS ; EUPLOTES-AEDICULATUS ; PARAMECIUM-BURSARIA ; PLAGIOPYLA-NASUTA ; HOLOSPORA-OBTUSA
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35112
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLi, Ming; Wang, Guitang
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Weishan,Li, Ming,Xiong, Fan,et al. Identification of Intracellular Bacteria in the Ciliate Balantidium ctenopharyngodoni (Ciliophora, Litostomatea)[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2020(1):10.
APA Zhao, Weishan.,Li, Ming.,Xiong, Fan.,Zhang, Dong.,Wu, Shangong.,...&Wang, Guitang.(2020).Identification of Intracellular Bacteria in the Ciliate Balantidium ctenopharyngodoni (Ciliophora, Litostomatea).JOURNAL OF EUKARYOTIC MICROBIOLOGY(1),10.
MLA Zhao, Weishan,et al."Identification of Intracellular Bacteria in the Ciliate Balantidium ctenopharyngodoni (Ciliophora, Litostomatea)".JOURNAL OF EUKARYOTIC MICROBIOLOGY .1(2020):10.
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