IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
A rapid and reliable method for identifying genetic sex in obscure pufferfish (Takifugu obscures)
Gao, Fan-Xiang1; Shi, Yan1; Duan, Wen1; Lu, Wei-Jia2; Huang, Wen3; Zhang, Xiao-Juan2; Zhao, Zhe1; Zhou, Li2; Gui, Jian-Fang2
2020-03-30
Source PublicationAQUACULTURE
ISSN0044-8486
Volume519Issue:1Pages:6
Abstract

Obscure pufferfish (Takifugu obscurus) is an important aquaculture species in China, and sex control breeding is extremely attractive in this species because the testes of male pufferfish are more popular and expensive. However, the sex-specific marker remains unknown and reliable genetic sex identification method has not been established, which has hampered the development of monosexual aquaculture. In this study, the sex-linked amhr2 SNP site of obscure pufferfish was firstly identified by sequencing and high-resolution melting (HRM) analysis. The males exhibit heterozygous genotype (C/G), while females have homozygous genotype (C/C), which is identical to its closely-related species (T. rubripes, T. poecilonotus and T. pardalis). Then, two pairs of male-specific primers were designed based on the sex-specific SNP site and a high-throughput sexing method was subsequently developed with benefits including high specificity and superb sensitivity. Particularly, this method has greatly reduced analysis time and costs compared to the previously established HRM analysis. These results will be beneficial to develop the sex control breeding in obscure pufferfish.

KeywordObscure pufferfish Takifugu obscurus Amhr2 Sex-specific marker Genetic sex identification Sex determination
DOI10.1016/j.aquaculture.2019.734749
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000511835000022
WOS KeywordDOMAIN-CONTAINING GENE ; DNA MELTING ANALYSIS ; EXTRA MICROCHROMOSOMES ; YELLOW CATFISH ; ALKALINE LYSIS ; MARKERS ; DIFFERENTIATION ; ESTRADIOL-17-BETA ; IDENTIFICATION
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35072
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhao, Zhe; Zhou, Li; Gui, Jian-Fang
Affiliation1.Hohai Univ, Coll Oceanog, Inst Marine Biol, Nanjing 210098, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Peoples R China
Recommended Citation
GB/T 7714
Gao, Fan-Xiang,Shi, Yan,Duan, Wen,et al. A rapid and reliable method for identifying genetic sex in obscure pufferfish (Takifugu obscures)[J]. AQUACULTURE,2020,519(1):6.
APA Gao, Fan-Xiang.,Shi, Yan.,Duan, Wen.,Lu, Wei-Jia.,Huang, Wen.,...&Gui, Jian-Fang.(2020).A rapid and reliable method for identifying genetic sex in obscure pufferfish (Takifugu obscures).AQUACULTURE,519(1),6.
MLA Gao, Fan-Xiang,et al."A rapid and reliable method for identifying genetic sex in obscure pufferfish (Takifugu obscures)".AQUACULTURE 519.1(2020):6.
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