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Function characterization and expression regulation of two different-sized 3 ' untranslated region-containing interferon genes from clone F of gibel carp Carassius auratus gibelio
Zang, Zhao-Xing1,2; Dan, Cheng1; Zhou, Li1,2,3; Zhang, Qi-Ya1,2,3,4; Gui, Jian-Fang1,2,3; Zhang, Yi-Bing1,2,3,4
2020-03-01
Source PublicationMOLECULAR IMMUNOLOGY
ISSN0161-5890
Volume119Issue:1Pages:18-26
Abstract

Fish interferon (IFN)-mediated antiviral innate immunity is the first line of defense against virus invasion. In the present study, we identify two fish IFN genes (here tentatively named IFNa and IFNc) with different-sized 3' UTRs from clone F strain of gibel carp Carassius auratus gibelio. Carp IFNa has a relatively short 3'UTR without AU-rich elements (AREs) but IFNc has a long one with 9 AREs. Functionally, carp IFNa and IFNc display significantly antiviral potential to viral infection, likely through induction of downstream IFN-stimulated genes (ISGs). Both carp IFN genes are induced by viral infection, poly(I:C) treatment and IRF3/7, which are ascribed to the IFN-sensitive response elements (ISRE) within their promoters. Carp IFN genes are also induced by each other and by themselves, indicating existence of a positive feedback loop in fish IFN-mediated antiviral immune response. Comparative analyses of 3'UTR-mediated expression regulation at mRNA and protein levels show that the ARE-containing 3'UTR of carp IFNc rather than the short 3'UTR of carp IFNa promotes mRNA decay but instead results in high-level protein expression, indicating that 3'UTR of fish IFN mRNAs might be a potential factor for regulation of IFN-mediated antiviral immune response. Considering a fact that a given protein function is largely related to its protein level, these results suggest that both promoter and 3'UTR contribute to the transcription and translation of fish IFN genes, thus shaping their eventually antiviral potential.

KeywordCarassius auratus gibelio Interferon 3 ' UTR Antiviral function Regulation of transcription and translation
DOI10.1016/j.molimm.2020.01.004
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Immunology
WOS SubjectBiochemistry & Molecular Biology ; Immunology
WOS IDWOS:000517657900003
WOS KeywordI IFNS ; ANTIVIRAL RESPONSE ; ZEBRAFISH IRF1 ; FISH ; DISTINCT ; IDENTIFICATION ; EVOLUTION ; BINDING ; CONSERVATION ; IFN-PHI-1
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35056
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, Yi-Bing
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
3.Chinese Acad Sci, Innovat Acad Seed Design, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zang, Zhao-Xing,Dan, Cheng,Zhou, Li,et al. Function characterization and expression regulation of two different-sized 3 ' untranslated region-containing interferon genes from clone F of gibel carp Carassius auratus gibelio[J]. MOLECULAR IMMUNOLOGY,2020,119(1):18-26.
APA Zang, Zhao-Xing,Dan, Cheng,Zhou, Li,Zhang, Qi-Ya,Gui, Jian-Fang,&Zhang, Yi-Bing.(2020).Function characterization and expression regulation of two different-sized 3 ' untranslated region-containing interferon genes from clone F of gibel carp Carassius auratus gibelio.MOLECULAR IMMUNOLOGY,119(1),18-26.
MLA Zang, Zhao-Xing,et al."Function characterization and expression regulation of two different-sized 3 ' untranslated region-containing interferon genes from clone F of gibel carp Carassius auratus gibelio".MOLECULAR IMMUNOLOGY 119.1(2020):18-26.
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