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Dietary Scenedesmus ovalternus improves disease resistance of overwintering gibel carp (Carassius gibelio) by alleviating toll-like receptor signaling activation
Xu, Wenjie1; Li, Hongyan1,2; Wu, Liyun1,2; Jin, Junyan1,3; Zhu, Xiaoming1,3; Han, Dong1,3; Liu, Haokun1,3; Yang, Yunxia1,3; Xu, Xudong1; Xie, Shouqi1,3
2020-02-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume97Issue:1Pages:351-358
Abstract

This study was conducted to investigate the effect of dietary Scenedesmus ovalternus on the growth and disease resistance of gibel carp (Carassius gibelio) during overwintering. Gibel carp (initial body weight: 90.39 +/- 0.33 g) were fed with diets containing 0% or 4% Scenedesmus ovalternus (DS0 and DS4) for 4 weeks during the early overwintering period, and then all fish were left unfed during the late overwintering period. A bacterial challenge test using Aeromonas hydrophila was subsequently conducted. The 4% Scenedesmus ovalternus diet had no effect on the growth of gibel carp (P > 0.05), but did improve the survival rate after the challenge (P <= 0.05). In the DS0 group, the bacterial challenge decreased the contents of complement 3 (C3), immunoglobulin M (IgM), interleukin 2 (IL2) and tumor necrosis factor alpha (TNF alpha) in fish (P < 0.05); in the DS4 group, the challenge increased total antioxidant capacity (T-AOC) and myeloperoxidase (MPO) activity but decreased IL2 and TNF alpha contents (P < 0.05). The activities of MPO and contents of C3, IgM and TNF alpha were higher in the DS4 group than that fed the DS0 diet after bacterial challenge (P < 0.05). Compared to pre challenge, the expression levels of toll like receptor 2 (TLR2), toll like receptor 3 (TLR3), toll like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), Toll/IL-1 receptor domain-containing adaptor protein (TIRAP), TIR-domain-containing adapter-inducing interferon 13 (TRIF), nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor a (Ix13a), transforming growth factor beta (TGF beta), interleukin 1 beta (IL1 beta), tumor necrosis factor alpha 1 (TNF alpha 1) and interleukin 10 (IL10) in the head kidney of gibel carp were induced after challenge (P < 0.05). Gibel carp fed the DS4 diet showed lower expression of TGF beta in head kidney before the challenge and lower expression of TLR2, TLR3, TLR4, TIRAP, TRIF, I kappa B alpha, TNF alpha 1, IL10 and TGF beta after the challenge than that fed the DS0 diet (P < 0.05). Overall, Scenedesmus ovalternus supplement enhanced the resistances of gibel carp against A. hydrophila after overwintering via the TLR signaling pathway.

KeywordGibel carp (Carassius gibelio) Scenedesmus ovalternus Overwintering Disease resistance
DOI10.1016/j.fsi.2019.12.065
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000513983700036
WOS KeywordGILTHEAD SEA BREAM ; IMMUNE-RESPONSES ; OXIDATIVE STRESS ; NILE TILAPIA ; AEROMONAS-HYDROPHILA ; CHLORELLA-VULGARIS ; COMPLEMENT-SYSTEM ; LABEO-ROHITA ; FISH-MEAL ; EXPRESSION
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35044
Collection藻类生物技术和生物能源研发中心_期刊论文
Corresponding AuthorJin, Junyan
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Xu, Wenjie,Li, Hongyan,Wu, Liyun,et al. Dietary Scenedesmus ovalternus improves disease resistance of overwintering gibel carp (Carassius gibelio) by alleviating toll-like receptor signaling activation[J]. FISH & SHELLFISH IMMUNOLOGY,2020,97(1):351-358.
APA Xu, Wenjie.,Li, Hongyan.,Wu, Liyun.,Jin, Junyan.,Zhu, Xiaoming.,...&Xie, Shouqi.(2020).Dietary Scenedesmus ovalternus improves disease resistance of overwintering gibel carp (Carassius gibelio) by alleviating toll-like receptor signaling activation.FISH & SHELLFISH IMMUNOLOGY,97(1),351-358.
MLA Xu, Wenjie,et al."Dietary Scenedesmus ovalternus improves disease resistance of overwintering gibel carp (Carassius gibelio) by alleviating toll-like receptor signaling activation".FISH & SHELLFISH IMMUNOLOGY 97.1(2020):351-358.
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