Dietary Scenedesmus ovalternus improves disease resistance of overwintering gibel carp (Carassius gibelio) by alleviating toll-like receptor signaling activation | |
Xu, Wenjie1; Li, Hongyan1,2; Wu, Liyun1,2; Jin, Junyan1,3![]() | |
2020-02-01 | |
Source Publication | FISH & SHELLFISH IMMUNOLOGY
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ISSN | 1050-4648 |
Volume | 97Issue:1Pages:351-358 |
Abstract | This study was conducted to investigate the effect of dietary Scenedesmus ovalternus on the growth and disease resistance of gibel carp (Carassius gibelio) during overwintering. Gibel carp (initial body weight: 90.39 +/- 0.33 g) were fed with diets containing 0% or 4% Scenedesmus ovalternus (DS0 and DS4) for 4 weeks during the early overwintering period, and then all fish were left unfed during the late overwintering period. A bacterial challenge test using Aeromonas hydrophila was subsequently conducted. The 4% Scenedesmus ovalternus diet had no effect on the growth of gibel carp (P > 0.05), but did improve the survival rate after the challenge (P <= 0.05). In the DS0 group, the bacterial challenge decreased the contents of complement 3 (C3), immunoglobulin M (IgM), interleukin 2 (IL2) and tumor necrosis factor alpha (TNF alpha) in fish (P < 0.05); in the DS4 group, the challenge increased total antioxidant capacity (T-AOC) and myeloperoxidase (MPO) activity but decreased IL2 and TNF alpha contents (P < 0.05). The activities of MPO and contents of C3, IgM and TNF alpha were higher in the DS4 group than that fed the DS0 diet after bacterial challenge (P < 0.05). Compared to pre challenge, the expression levels of toll like receptor 2 (TLR2), toll like receptor 3 (TLR3), toll like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), Toll/IL-1 receptor domain-containing adaptor protein (TIRAP), TIR-domain-containing adapter-inducing interferon 13 (TRIF), nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor a (Ix13a), transforming growth factor beta (TGF beta), interleukin 1 beta (IL1 beta), tumor necrosis factor alpha 1 (TNF alpha 1) and interleukin 10 (IL10) in the head kidney of gibel carp were induced after challenge (P < 0.05). Gibel carp fed the DS4 diet showed lower expression of TGF beta in head kidney before the challenge and lower expression of TLR2, TLR3, TLR4, TIRAP, TRIF, I kappa B alpha, TNF alpha 1, IL10 and TGF beta after the challenge than that fed the DS0 diet (P < 0.05). Overall, Scenedesmus ovalternus supplement enhanced the resistances of gibel carp against A. hydrophila after overwintering via the TLR signaling pathway. |
Keyword | Gibel carp (Carassius gibelio) Scenedesmus ovalternus Overwintering Disease resistance |
DOI | 10.1016/j.fsi.2019.12.065 |
Indexed By | SCI |
Language | 英语 |
WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS ID | WOS:000513983700036 |
WOS Keyword | GILTHEAD SEA BREAM ; IMMUNE-RESPONSES ; OXIDATIVE STRESS ; NILE TILAPIA ; AEROMONAS-HYDROPHILA ; CHLORELLA-VULGARIS ; COMPLEMENT-SYSTEM ; LABEO-ROHITA ; FISH-MEAL ; EXPRESSION |
Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/35044 |
Collection | 藻类生物技术和生物能源研发中心_期刊论文 |
Corresponding Author | Jin, Junyan |
Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China |
Recommended Citation GB/T 7714 | Xu, Wenjie,Li, Hongyan,Wu, Liyun,et al. Dietary Scenedesmus ovalternus improves disease resistance of overwintering gibel carp (Carassius gibelio) by alleviating toll-like receptor signaling activation[J]. FISH & SHELLFISH IMMUNOLOGY,2020,97(1):351-358. |
APA | Xu, Wenjie.,Li, Hongyan.,Wu, Liyun.,Jin, Junyan.,Zhu, Xiaoming.,...&Xie, Shouqi.(2020).Dietary Scenedesmus ovalternus improves disease resistance of overwintering gibel carp (Carassius gibelio) by alleviating toll-like receptor signaling activation.FISH & SHELLFISH IMMUNOLOGY,97(1),351-358. |
MLA | Xu, Wenjie,et al."Dietary Scenedesmus ovalternus improves disease resistance of overwintering gibel carp (Carassius gibelio) by alleviating toll-like receptor signaling activation".FISH & SHELLFISH IMMUNOLOGY 97.1(2020):351-358. |
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