IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Effects of Bacillus licheniformis on the growth, antioxidant capacity, intestinal barrier and disease resistance of grass carp (Ctenopharyngodon idella)
Qin, Lu1,2,3; Xiang, Jinhua1,2,3; Xiong, Fan1,2; Wang, Guitang1,2,3; Zou, Hong1,2,3; Li, Wenxiang1,2,3; Li, Ming1,2,3; Wu, Shangong1,2,3
2020-02-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume97Issue:1Pages:344-350
Abstract

To study the effect of dietary supplementation of Bacillus licheniformis FA6 on the growth, survival and intestinal health of grass carp, we assessed the antioxidant capacity, intestinal barrier, expression levels of immune genes, and the resistance to Aeromonas hydrophila AH-1 infection. Experimental setup comprised three groups (90 specimens each; average initial weight = 16.5 g): the control group was fed the basal diet without B. licheniformis, the low-dose (LD) group was supplemented with B. licheniformis at the concentration of 1 x 10(5) cfu/g, and the high-dose (HD) group with 1 x 10(6) cfu/g. After 56 days of growth trial, the challenge test with A. hydrophila AH-1 was conducted for 14 days. The results revealed that the grass carp in LD group and HD group had significantly (p < 0.05) improved percent weight gain (PWG) and specific growth rate (SGR) parameters. Additionally, the antioxidant status was improved, which included increased superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) levels in the serum, and upregulated mRNA levels of antioxidant enzymes MnSOD and catalase (CAT) in the intestine. Meanwhile, B. licheniforrnis FA6 supplementation groups exhibited a decreased mRNA expression of proinflammatory cytokines (such as IL-1 beta and TNF-alpha) and increased the expression of anti-inflammatory cytokine IL-10. Histological (villi length was increased) and gene expression (qPCR: upregulated ZO-1, occludin, and claudin-c) analyses suggested improved functioning of the intestinal barrier. Post-challenge mortality rates in LD and HD groups were significantly lower (56.6% and 70% respectively) than in the control group (100%). Overall, these results indicated that dietary supplementation of B. licheniformis FA6 can improve growth, antioxidant capacity, intestinal barrier functions and disease resistance of grass carp.

KeywordBacillus licheniformis Grass carp Ctenopharyngodon idella Growth Antioxidant capacity Intestinal barrier Disease resistance
DOI10.1016/j.fsi.2019.12.040
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000513983700035
WOS KeywordNF-KAPPA-B ; DIETARY SUPPLEMENTATION ; PHYSICAL BARRIER ; GENE-EXPRESSION ; IMMUNE-RESPONSE ; PERFORMANCE ; MORPHOLOGY ; MICROBIOTA ; SUBTILIS ; IMPROVES
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35032
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWu, Shangong
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Qin, Lu,Xiang, Jinhua,Xiong, Fan,et al. Effects of Bacillus licheniformis on the growth, antioxidant capacity, intestinal barrier and disease resistance of grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2020,97(1):344-350.
APA Qin, Lu.,Xiang, Jinhua.,Xiong, Fan.,Wang, Guitang.,Zou, Hong.,...&Wu, Shangong.(2020).Effects of Bacillus licheniformis on the growth, antioxidant capacity, intestinal barrier and disease resistance of grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,97(1),344-350.
MLA Qin, Lu,et al."Effects of Bacillus licheniformis on the growth, antioxidant capacity, intestinal barrier and disease resistance of grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 97.1(2020):344-350.
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