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Membrane IgM(+) plasma cells in grass carp (Ctenopharyngodon idella): Insights into the conserved evolution of IgM(+) plasma cells in vertebrates
Cui, Zheng-Wei1,3,4; Zhang, Xiang-Yang4; Wu, Chang-Song1; Zhang, Yong-An1,2,4; Zhou, Yang1; Zhang, Xu-Jie1
2020-05-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume106Issue:1Pages:10
Abstract

Teleost fish are the most primitive bony vertebrates that contain B cells; thus, comparative analysis of teleost naive/mature B cells and plasma cells can provide helpful evidence for understanding the evolution paradigms of these two B-cell subpopulations in vertebrates. In this study, we developed monoclonal antibody against grass carp IgM and identified two different IgM(+) cell subsets: IgM(+) lymphocytes (Lym), resembling naive/mature B cells, and IgM(+) myeloid cells (Mye), resembling plasma cells. Like plasma cells in mammals, the size of IgM(+) Mye is significantly larger than that of IgM(+) Lym, as revealed by flow cytometric analysis and transmission electron microscopy. The IgM(+) Mye were further verified as plasma cells because they showed gene expression patterns similar with those of human plasma cells and a great capacity to secrete IgM. Like mammalian IgM(+) and IgA(+) plasma cells, not IgG(+) plasma cells, grass carp IgM(+) Mye also expressed membrane immunoglobulins, a feature conserved in IgM(+) plasma cells in vertebrates. Furthermore, recombinant CD4OL or IL-21 alone could induce the plasma cell generation and IgM secretion, while the combination of CD4OL and IL-21 had greater effect on IgM secretion, but not on plasma cell generation. This study fills an important gap in the knowledge of plasma cells in teleost fish and provides critical insights into the conserved evolution of IgM(+) plasma cells in vertebrates.

KeywordIgM(+) B cell Plasma cell CD4OL IL-21 Grass carp
DOI10.1016/j.dci.2020.103613
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000517853000009
WOS KeywordMEMORY B ; PERIPHERAL-BLOOD ; DIFFERENTIATION ; IL-21 ; EXPRESSION ; BLIMP-1 ; IDENTIFICATION ; LYMPHOCYTES ; APOPTOSIS ; NAIVE
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/35013
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorZhou, Yang; Zhang, Xu-Jie
Affiliation1.Huazhong Agr Univ, Coll Fisheries, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Chinese Acad Fishery Sci, Key Lab Fishery Drug Dev, Minist Agr China, River Fisheries Res Inst, Guangzhou 510380, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Cui, Zheng-Wei,Zhang, Xiang-Yang,Wu, Chang-Song,et al. Membrane IgM(+) plasma cells in grass carp (Ctenopharyngodon idella): Insights into the conserved evolution of IgM(+) plasma cells in vertebrates[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,106(1):10.
APA Cui, Zheng-Wei,Zhang, Xiang-Yang,Wu, Chang-Song,Zhang, Yong-An,Zhou, Yang,&Zhang, Xu-Jie.(2020).Membrane IgM(+) plasma cells in grass carp (Ctenopharyngodon idella): Insights into the conserved evolution of IgM(+) plasma cells in vertebrates.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,106(1),10.
MLA Cui, Zheng-Wei,et al."Membrane IgM(+) plasma cells in grass carp (Ctenopharyngodon idella): Insights into the conserved evolution of IgM(+) plasma cells in vertebrates".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 106.1(2020):10.
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