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Molecular phylogenetics and mitogenomics of three avian dicrocoeliids (Digenea: Dicrocoeliidae) and comparison with mammalian dicrocoeliids
Suleman1,2; Khan, Mian Sayed2; Tkach, Vasyl V.3; Muhammad, Nehaz1; Zhang, Dong4,5; Zhu, Xing-Quan1,6; Ma, Jun1
Source PublicationPARASITES & VECTORS

BackgroundThe Dicrocoeliidae are digenetic trematodes mostly parasitic in the bile ducts and gall bladder of various avian and mammalian hosts. Until recently their systematics was based on morphological data only. Due to the high morphological uniformity across multiple dicrocoeliid taxa and insufficient knowledge of relative systematic value of traditionally used morphological characters, their taxonomy has always been unstable. Therefore, DNA sequence data provide a critical independent source of characters for phylogenetic inference and improvement of the system.MethodsWe examined the phylogenetic affinities of three avian dicrocoeliids representing the genera Brachylecithum, Brachydistomum and Lyperosomum, using partial sequences of the nuclear large ribosomal subunit (28S) RNA gene. We also sequenced the complete or nearly complete mitogenomes of these three isolates and conducted a comparative mitogenomic analysis with the previously available mitogenomes from three mammalian dicrocoeliids (from 2 different genera) and examined the phylogenetic position of the family Dicrocoeliidae within the order Plagiorchiida based on concatenated nucleotide sequences of all mitochondrial genes (except trnG and trnE).ResultsCombined nucleotide diversity, Kimura-2-parameter distance, non-synonymous/synonymous substitutions ratio and average sequence identity analyses consistently demonstrated that cox1, cytb, nad1 and two rRNAs were the most conserved and atp6, nad5, nad3 and nad2 were the most variable genes across dicrocoeliid mitogenomes. Phylogenetic analyses based on mtDNA sequences did not support the close relatedness of the Paragonimidae and Dicrocoeliidae and suggested non-monophyly of the Gorgoderoidea as currently recognized.ConclusionsOur results show that fast-evolving mitochondrial genes atp6, nad5 and nad3 would be better markers than slow-evolving genes cox1 and nad1 for species discrimination and population level studies in the Dicrocoeliidae. Furthermore, the Dicrocoeliidae being outside of the clade containing other xiphidiatan trematodes suggests a need for the re-evaluation of the taxonomic content of the Xiphidiata.

KeywordDicrocoeliidae Nucleotide diversity Mitochondrial genomes Molecular phylogeny
Indexed BySCI
WOS Research AreaParasitology ; Tropical Medicine
WOS SubjectParasitology ; Tropical Medicine
WOS IDWOS:000514912800002
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorTkach, Vasyl V.; Zhu, Xing-Quan; Ma, Jun
Affiliation1.Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou Vet Res Inst, Lanzhou 730046, Gansu, Peoples R China
2.Univ Swabi, Dept Zool, Swabi 23340, Khyber Pakhtunk, Pakistan
3.Univ North Dakota, Dept Biol, Grand Forks, ND 58202 USA
4.Chinese Acad Sci, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China
5.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
6.Yangzhou Univ, Coll Vet Med, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Suleman,Khan, Mian Sayed,Tkach, Vasyl V.,et al. Molecular phylogenetics and mitogenomics of three avian dicrocoeliids (Digenea: Dicrocoeliidae) and comparison with mammalian dicrocoeliids[J]. PARASITES & VECTORS,2020,13(1):12.
APA Suleman.,Khan, Mian Sayed.,Tkach, Vasyl V..,Muhammad, Nehaz.,Zhang, Dong.,...&Ma, Jun.(2020).Molecular phylogenetics and mitogenomics of three avian dicrocoeliids (Digenea: Dicrocoeliidae) and comparison with mammalian dicrocoeliids.PARASITES & VECTORS,13(1),12.
MLA Suleman,et al."Molecular phylogenetics and mitogenomics of three avian dicrocoeliids (Digenea: Dicrocoeliidae) and comparison with mammalian dicrocoeliids".PARASITES & VECTORS 13.1(2020):12.
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