IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Single-cell transcriptome sequencing of rumen ciliates provides insight into their molecular adaptations to the anaerobic and carbohydrate-rich rumen microenvironment
Feng, Jin-Mei1; Jiang, Chuan-Qi2,3,4,5; Sun, Zong-Yi5; Hua, Cong-Jie1; Wen, Jian-Fan6; Miao, Wei5,7,8; Xiong, Jie5
2020-02-01
Source PublicationMOLECULAR PHYLOGENETICS AND EVOLUTION
ISSN1055-7903
Volume143Issue:1Pages:12
Abstract

Rumen ciliates are a specialized group of ciliates exclusively found in the anaerobic, carbohydrate-rich rumen microenvironment. However, the molecular and mechanistic basis of the physiological and behavioral adaptation of ciliates to the rumen microenvironment is undefined. We used single-cell transcriptome sequencing to explore the adaptive evolution of three rumen ciliates: two entodiniomorphids, Entodinium furca and Diplodinium dentatum; and one vestibuliferid, Isotricha intestinalis. We found that all three species are members of monophyletic orders within the class Litostomatea, with E. furca and D. dentatum in Entodiniomorphida and I. intestinalis in Vestibuliferida. The two entodiniomorphids might use H-2-producing mitochondria and the vestibuliferid might use anaerobic mitochondria to survive under strictly anaerobic conditions. Moreover, carbohydrate-active enzyme (CAZyme) genes were identified in all three species, including cellulases, hemicellulases, and pectinases. The evidence that all three species have acquired prokaryote-derived genes by horizontal gene transfer (HGT) to digest plant biomass includes a significant enrichment of gene ontology categories such as cell wall macromolecule catabolic process and carbohydrate catabolic process and the identification of genes in common between CAZyme and HGT groups. These findings suggest that HGT might be an important mechanism in the adaptive evolution of ciliates to the rumen microenvironment.

KeywordRumen ciliate Adaptive evolution Phylogenomics Rumen microenvironment Horizontal gene transfer
DOI10.1016/j.ympev.2019.106687
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation
Indexed BySCI
Language英语
Funding ProjectNatural Science Foundation of China[31501854] ; Natural Science Foundation of China[91631303] ; Hubei Province Health and Family Planning Scientific Research Project of China[WJ2017X015] ; China Postdoctoral Science Foundation[2018M642954]
WOS Research AreaBiochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS IDWOS:000506225200017
WOS KeywordHORIZONTAL GENE-TRANSFER ; CODON USAGE BIAS ; PHYLOGENETIC ANALYSES ; PROTOZOAN ; HYDROGENOSOMES ; EVOLUTION ; GENOME ; RECONSTRUCTION ; PROTEOME ; BACTERIA
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Hubei Province Health and Family Planning Scientific Research Project of China ; 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Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34962
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorMiao, Wei; Xiong, Jie
Affiliation1.Jianghan Univ, Sch Med, Dept Pathogen Biol, Wuhan 430056, Peoples R China
2.Shenzhen Inst Guangdong Ocean Univ, Shenzhen 518120, Peoples R China
3.Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen 518120, Peoples R China
4.Shenzhen Dapeng New Dist Sci & Technol Innovat Se, Shenzhen 518119, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
6.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Yunnan, Peoples R China
7.State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
8.CAS Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Feng, Jin-Mei,Jiang, Chuan-Qi,Sun, Zong-Yi,et al. Single-cell transcriptome sequencing of rumen ciliates provides insight into their molecular adaptations to the anaerobic and carbohydrate-rich rumen microenvironment[J]. MOLECULAR PHYLOGENETICS AND EVOLUTION,2020,143(1):12.
APA Feng, Jin-Mei.,Jiang, Chuan-Qi.,Sun, Zong-Yi.,Hua, Cong-Jie.,Wen, Jian-Fan.,...&Xiong, Jie.(2020).Single-cell transcriptome sequencing of rumen ciliates provides insight into their molecular adaptations to the anaerobic and carbohydrate-rich rumen microenvironment.MOLECULAR PHYLOGENETICS AND EVOLUTION,143(1),12.
MLA Feng, Jin-Mei,et al."Single-cell transcriptome sequencing of rumen ciliates provides insight into their molecular adaptations to the anaerobic and carbohydrate-rich rumen microenvironment".MOLECULAR PHYLOGENETICS AND EVOLUTION 143.1(2020):12.
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