IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Stable Genome Incorporation of Sperm-derived DNA Fragments in Gynogenetic Clone of Gibel Carp
Chen, Fan1,2; Li, Xi-Yin1,2; Zhou, Li1,2; Yu, Peng1,2; Wang, Zhong-Wei1,2; Li, Zhi1; Zhang, Xiao-Juan1; Wang, Yang1,2; Gui, Jian-Fang1,2
2020-01-04
Source PublicationMARINE BIOTECHNOLOGY
ISSN1436-2228
Issue1Pages:13
Abstract

How unisexual animals eliminate deleterious mutations to avoid dead ends is one of the most interesting puzzles in evolutionary genetics. Incorporation of microchromosomes derived from exogenous sperm had been observed in gynogenetic animals, but little is known about their detailed process and hereditary fate. Here, we show a stable genome incorporation case in an artificial clone F of gynogenetic gibel carp (Carassius gibelio). A total of 12 exogenous DNA fragments were screened through a read depth-dependent comparison strategy and confirmed to be specific to the clone F and the paternal blunt snout bream (Megalobrama amblycephala Yin) by SCAR (sequence characterized amplified regions) marker detection. Moreover, these sperm-derived DNA fragments were not detected in some samples in early gynogenetic generations, but they were found to exist in all examined individuals through artificial gynogenetic selections of 13 generations, implying that they might have stably incorporated into the genome of clone F. Furthermore, chromosome localization and sequence characterization indicate that the largest fragment CgA22_34 is derived from blunt snout bream non-LTR retrotransposon and durably incorporated into only one of three homologous chromosomes of gibel carp clone F. Our results suggest that the incorporated sperm-derived DNA fragments by allogynogenesis should increase genetic diversity and introduce new traits into unisexual animals which will benefit genetic breeding of gibel carp. During the process, transposable elements (TEs) may play significant roles in shaping the genome structures. Simultaneously, the incorporated DNA fragments are able to be used as genetic markers to perform selective breeding programs in aquaculture practices of gibel carp.

KeywordGynogenesis Unisexual reproduction Genome incorporation Transposable element Marker-assisted selection breeding
DOI10.1007/s10126-019-09930-w
Funding OrganizationStrategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation
Indexed BySCI
Language英语
Funding ProjectStrategic Priority Research Program of CAS, China Agriculture Research System[CARS-45-07] ; National Natural Science Foundation[31772839]
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000505409800001
WOS KeywordUNISEXUAL SALAMANDERS ; TRANSPOSABLE ELEMENTS ; MOBILITY PATHWAYS ; SEX DETERMINATION ; GENUS AMBYSTOMA ; GENETIC-BASIS ; REVEALS ; MICROCHROMOSOMES ; REPRODUCTION ; CHROMOSOME
PublisherSPRINGER
Funding OrganizationStrategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; Strategic Priority Research Program of CAS, China Agriculture Research System ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34959
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, Yang; Gui, Jian-Fang
Affiliation1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Chen, Fan,Li, Xi-Yin,Zhou, Li,et al. Stable Genome Incorporation of Sperm-derived DNA Fragments in Gynogenetic Clone of Gibel Carp[J]. MARINE BIOTECHNOLOGY,2020(1):13.
APA Chen, Fan.,Li, Xi-Yin.,Zhou, Li.,Yu, Peng.,Wang, Zhong-Wei.,...&Gui, Jian-Fang.(2020).Stable Genome Incorporation of Sperm-derived DNA Fragments in Gynogenetic Clone of Gibel Carp.MARINE BIOTECHNOLOGY(1),13.
MLA Chen, Fan,et al."Stable Genome Incorporation of Sperm-derived DNA Fragments in Gynogenetic Clone of Gibel Carp".MARINE BIOTECHNOLOGY .1(2020):13.
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