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Effect of recycling the culture medium on biodiversity and population dynamics of bio-contaminants in Spirulina platensis mass culture systems
Yuan, Danni1,2; Yao, Mimi1,2; Wang, Lan1,2; Li, Yanhua1,2; Gong, Yingchun1,2; Hu, Qiang1,2,3,4,5
2019-12-01
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume44Issue:1Pages:10
Abstract

Spirulina (Arthrospira) is an important microalga that can generate a variety of commercial products. A semi-continuous mode of cultivation is often employed in microalgal cultivation. The traditional view, however, has been that cultures with recycled medium are more likely to be contaminated by microzooplankton, which is regarded as one of the critical problems in mass algal cultivation. In this study, the relationship between the population dynamics of contaminants and the medium conditions was investigated in Spirulina cultures. Spirulina platensis was cultivated in three groups of raceway ponds: one with fresh culture medium, one with medium that had been recycled for a month, and the third with medium recycled for six months. The results showed that totally 13 species of microzooplankton were observed by light microscopy, and 42 operational taxonomic units (OTUs) of prokaryotic contaminants were detected using amplicon sequencing. Out of all the contaminants, Brachionus plicatilis and Euplaesiobystra hypersalinica were observed to be the most harmful species in Spirulina cultures, while Proteobacteria were the most commonly found non-Cyanobacteria OTUs. On the initial day, more species of microzooplankton were introduced to the cultures that had recycled medium (9 species) than to those that had fresh medium (5 species). By the end of the cultivation, the algal biomass in the fresh medium group was the highest (2.8 g L-1), being almost 5 times higher than in the other two groups (around 0.50 g L-1). Our results proved that Spirulina can grow the best with fresh medium and that the more the culture medium is recycled, the stronger the inhibition on the growth of microalgae and microzooplankton. In order to improve large-scale Spirulina production, it is necessary to both subject the recycled medium to appropriate treatment to reduce the presence of harmful predators and to find effective ways to control contaminants.

KeywordSpirulina cultivation Microzooplankton Amplicon sequencing Recycled medium
DOI10.1016/j.algal.2019.101718
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000498792900023
WOS KeywordMICROZOOPLANKTON ; CULTIVATION ; SCENEDESMUS ; INDUSTRY ; ALGAE ; WATER ; LAKES
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34835
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorGong, Yingchun; Hu, Qiang
Affiliation1.Chinese Acad Sci, Ctr Microalgal Biotechnol & Biofuels, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
3.SDIC Biotech Investment Co LTD, SDIC Microalgae Biotechnol Ctr, Beijing 065200, Peoples R China
4.Beijing Key Lab Algae Biomass, Beijing 100142, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Yuan, Danni,Yao, Mimi,Wang, Lan,et al. Effect of recycling the culture medium on biodiversity and population dynamics of bio-contaminants in Spirulina platensis mass culture systems[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2019,44(1):10.
APA Yuan, Danni,Yao, Mimi,Wang, Lan,Li, Yanhua,Gong, Yingchun,&Hu, Qiang.(2019).Effect of recycling the culture medium on biodiversity and population dynamics of bio-contaminants in Spirulina platensis mass culture systems.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,44(1),10.
MLA Yuan, Danni,et al."Effect of recycling the culture medium on biodiversity and population dynamics of bio-contaminants in Spirulina platensis mass culture systems".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 44.1(2019):10.
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