IHB OpenIR  > 藻类生物技术和生物能源研发中心  > 期刊论文
Unraveling enhanced membrane lipid biosynthesis in Chlamydomonas reinhardtii starchless mutant sta6 by using an electrospray ionization mass spectrometry-based lipidomics method
Zang Zhengrong1,2,3; Li Yanhua1,2; Hu Qiang1,2,4,5,6; Han Danxiang1,2
2019-11-27
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Issue1Pages:12
Abstract

The unicellular green alga Chlamydomonas reinhardtii, a well-established model organism, has been widely used in dissecting glycerolipid metabolism in oxygenating photosynthetic organisms. In previous studies, it has been found that shunting carbon precursors from the starch synthesis pathway can lead to a 10-fold increase in TAG content as compared to the wild type, but it is unknown whether inactivation of AGPase may affect membrane lipids biosynthesis. The study aims to investigate global changes in lipid metabolism and homeostasis in the starchless mutant C. reinhardtii sta6. By utilizing an electrospray ionization/mass spectrometry (ESI/MS)-based lipidomics approach, a total of 105 membrane lipid molecules of C. reinhardtii were resolved, including 16 monogalactosyldiacylglycerol (MGDG), 16 digalactosyldiacylglycerol (DGDG), 11 phosphatidylglycerol (PG), 6 sulfoquinovosyldiacylglycerol (SQDG), 49 diacylglyceryl-N,N,N-trimethylhomoserine (DGTS), 2 phosphatidylethanolamine (PE), and 5 phosphatidylinositol (PI) molecules. The quantitative results indicated that the membrane lipid profiles were similar between the two C. reinhardtii strains grown under both low- and high-light conditions, but the cellular contents of a great number of lipids were altered in sta6 due to the defect in starch biosynthesis. Under low-light conditions, sta6 accumulated more PI, MGDG, DGDG but less amounts of DGTS as compared to WT. Under high light, sta6 cells contained higher content membrane lipids than cc-124, except for PG, which is more or less similar in both strains. Our results demonstrate that the cellular membrane lipid homeostasis underwent profound changes in the starchless mutant, and thereby its physiological impact remains to be explored.

KeywordChlamydomonas reinhardtii chloroplast lipidomes extraplastidic lipidomes electrospray ionization mass spectrometry
DOI10.1007/s00343-019-9138-1
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000500379800003
WOS KeywordFATTY-ACIDS ; POSITIONAL DISTRIBUTION ; NITROGEN STARVATION ; CRUDE EXTRACTS ; METABOLISM ; TRIACYLGLYCEROL ; GLYCEROLIPIDS ; CHEMISTRY ; CHAIN
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34826
Collection藻类生物技术和生物能源研发中心_期刊论文
Corresponding AuthorHan Danxiang
Affiliation1.Chinese Acad Sci, Ctr Microalgal Biotechnol & Biofuels, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
6.SDIC Biotech Investment Co Ltd, Beijing Key Lab Algae Biomass, State Dev & Investment Corp, Microalgae Biotechnol Ctr, Beijing 100142, Peoples R China
Recommended Citation
GB/T 7714
Zang Zhengrong,Li Yanhua,Hu Qiang,et al. Unraveling enhanced membrane lipid biosynthesis in Chlamydomonas reinhardtii starchless mutant sta6 by using an electrospray ionization mass spectrometry-based lipidomics method[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019(1):12.
APA Zang Zhengrong,Li Yanhua,Hu Qiang,&Han Danxiang.(2019).Unraveling enhanced membrane lipid biosynthesis in Chlamydomonas reinhardtii starchless mutant sta6 by using an electrospray ionization mass spectrometry-based lipidomics method.JOURNAL OF OCEANOLOGY AND LIMNOLOGY(1),12.
MLA Zang Zhengrong,et al."Unraveling enhanced membrane lipid biosynthesis in Chlamydomonas reinhardtii starchless mutant sta6 by using an electrospray ionization mass spectrometry-based lipidomics method".JOURNAL OF OCEANOLOGY AND LIMNOLOGY .1(2019):12.
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