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Fish herpesvirus protein (CaHV-138L) can target to mitochondrial protein FoF1 ATPase
Zhao, Yu-Han1; Zeng, Xiao-Tao1; Zhang, Qi-Ya1,2
Source PublicationVIRUS RESEARCH

Herpesvirus infection usually relies on the interaction between viral protein and host protein to enhance replication of the enveloped virus. Fish Carassius auratus herpesvirus (CaHV) is highly pathogenic pathogen causing gill acute hemorrhages of crucian carp (Carassius auratus) and high moritality rates among those infected fish. The protein of CaHV (CaHV-138 L) containing two transmembrane (TM) domains and an immunoglobulin C-2 Type (IGc2) domain was predicted as a viral membrane protein. In this investigation, fluorescence observation showed that full-length CaHV-138 L mainly localized on the plasma membrane or around nuclear membrane of fish fathead minnow (FHM) cells in a punctate pattern. The TM domain deletion mutants of CaHV-138 L (Delta TM1, Delta TM2, and Delta TM1 &Delta TM2) diffusely distributed in both the cytoplasm and the nucleus, mainly presented patchy fashion in the cytoplasm, and mainly presented both in the nucleus and in the cytoplasm, respectively. Obviously, the TM domain deletion mutants significantly affected CaHV-138 L subcellular localization. Meanwhile, colocalization assay showed that the full-length viral protein colocalized with mitochondria. Furthermore, the interaction between CaHV-138 L and host protein was identified by yeast two-hybrid (Y2H) and co-immunoprecipitation (co-IP) assays. The host mitochondrial protein FoF1 ATP synthase (FoF1-ATPase) that interacts with this viral protein was screened. The data indicated that CaHV-138 L can target to mitochondrial protein FoF1-ATPase, which might provide energy for virus replication through mediating mitochondrial ATP synthesis. This study has provided valuable information for better understanding of the links of herpesvirus proteins with aquaculture animal proteins.

KeywordCarassius auratus herpesvirus (CaHV) Viral protein Mitochondrial FoF1-ATPase Virus-host interaction Yeast two hybrid (Y2H) Co-immunoprecipitation (co-IP) assays
Indexed BySCI
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000500373700003
Citation statistics
Document Type期刊论文
Corresponding AuthorZhang, Qi-Ya
Affiliation1.Univ Chinese Acad Sci, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Yu-Han,Zeng, Xiao-Tao,Zhang, Qi-Ya. Fish herpesvirus protein (CaHV-138L) can target to mitochondrial protein FoF1 ATPase[J]. VIRUS RESEARCH,2020,275(1):7.
APA Zhao, Yu-Han,Zeng, Xiao-Tao,&Zhang, Qi-Ya.(2020).Fish herpesvirus protein (CaHV-138L) can target to mitochondrial protein FoF1 ATPase.VIRUS RESEARCH,275(1),7.
MLA Zhao, Yu-Han,et al."Fish herpesvirus protein (CaHV-138L) can target to mitochondrial protein FoF1 ATPase".VIRUS RESEARCH 275.1(2020):7.
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