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Grass carp cGASL negatively regulates fish IFN response by targeting MITA
Zhou, Yu1,4; Lu, Long-Feng1; Lu, Xiao-Bing1,4; Li, Shun1; Zhang, Yong-An1,2,3
2019-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume94Issue:1Pages:871-879
Abstract

Mammalian cyclic GMP-AMP synthase (cGAS) senses double-stranded (ds) DNA in the cytosol to activate the innate antiviral response. In the present study, a cGAS-like gene, namely cGASL, was cloned from grass carp Ctenopharyngodon idellus, and its role as a negative regulator of the IFN response was revealed. Phylogenetic analysis indicated that cGASL was evolutionarily closest to cGAS, but was not a true ortholog of cGAS. Overexpression of cGASL inhibited poly LC-stimulated grass carp (gc)IFN1pro and ISRE activities. In addition, MITA-, but not TBK1-mediated activation of gclFNlpro was impaired by cGASL. Co-immunoprecipitation and Western blot experiments indicated that cGASL interacted with MITA and TBK1, resulting in a reduction in the phosphorylation of MITA. Lastly, overexpression of cGASL reduced the transcriptional levels of several IFNstimulated genes activated by MITA. Collectively, these data suggest that cGASL is a negative regulator of IFN response by targeting MITA in fish.

KeywordcGAS-like Negative regulator Interferon MITA Grass carp
DOI10.1016/j.fsi.2019.10.010
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000496892400092
WOS KeywordGMP-AMP SYNTHASE ; DNA SENSOR CGAS ; RIG-I ; PROTEIN ; IRF3 ; REQUIREMENT ; EXPRESSION ; RECEPTORS ; PATHWAY ; MAB21L2
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34798
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorLi, Shun; Zhang, Yong-An
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Yu,Lu, Long-Feng,Lu, Xiao-Bing,et al. Grass carp cGASL negatively regulates fish IFN response by targeting MITA[J]. FISH & SHELLFISH IMMUNOLOGY,2019,94(1):871-879.
APA Zhou, Yu,Lu, Long-Feng,Lu, Xiao-Bing,Li, Shun,&Zhang, Yong-An.(2019).Grass carp cGASL negatively regulates fish IFN response by targeting MITA.FISH & SHELLFISH IMMUNOLOGY,94(1),871-879.
MLA Zhou, Yu,et al."Grass carp cGASL negatively regulates fish IFN response by targeting MITA".FISH & SHELLFISH IMMUNOLOGY 94.1(2019):871-879.
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