In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill | |
Zhang, Delu1; Liu, Siyi1; Zhang, Jing2; Zhang, Jian Kong1; Hu, Chunxiang3; Liu, Yongding3 | |
2016-08-01 | |
Source Publication | AQUATIC TOXICOLOGY
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ISSN | 0166-445X |
Volume | 177Issue:1Pages:484-493 |
Abstract | Aphantoxins, neurotoxins or paralytic shellfish poisons (PSPs) generated by Aphanizomenon flos-aquae, are a threat to environmental safety and human health in eutrophic waters worldwide. The molecular mechanisms of neurotoxin function have been studied; however, the effects of these neurotoxins on oxidative stress, ion transport, gas exchange, and branchial ultrastructure in fish gills are not fully understood. Aphantoxins extracted from A. flos-aquae DC-1 were detected by high-performance liquid chromatography. The major ingredients were gonyautoxins 1 and 5 and neosaxitoxin, which comprised 34.04%, 21.28%, and 12.77% of the total, respectively. Zebrafish (Danio rerio) were administered A. fibs aquae DC-1 aphantoxins at 5.3 or 7.61 mu g saxitoxin equivalents (eq)/kg (low and high doses, respectively) by intraperitoneal injection. The activities of Na+-K+-ATPase (NKA), carbonic anhydrase (CA), and lactate dehydrogenase (LDH), ultrastructural alterations in chloride and epithelial cells, and reactive oxygen species (ROS) and total antioxidative capacity (T-AOC) were investigated in the gills during the first 24 h after exposure. Aphantoxins significantly increased the level of ROS and decreased the T-AOC in zebrafish gills from 3 to 12 h post-exposure, suggesting an induction of oxidative stress and inhibition of antioxidant capacity. Reduced activities of NKA and CA demonstrated abnormal ion transport and gas exchange in the gills of aphantoxin-treated fish. Toxin administration also resulted in increased LDH activity and ultrastructural alterations in chloride and epithelial cells, suggesting a disruption of function and structure in zebrafish gills. The observed abnormalities in zebrafish gills occurred in a time-and dose-dependent manner. These findings demonstrate that aphantoxins or PSPs may inhibit ion transport and gas exchange, increase LDH activity, and result in ultrastructural damage to the gills through elevations in oxidative stress and reduced antioxidant capacity. These effects of aphantoxins in the gills of zebrafish suggest an induction of respiratory toxicity. The parameters investigated in this study may be also considered as biomarkers for studying aphantoxin/PSP exposure and cyanobacterial blooms in nature. (C) 2016 Elsevier B.V. All rights reserved. |
Keyword | Carbonic anhydrase Cyanobacterial neurotoxin Na+-K+-ATPase Respiratory toxicity Zebrafish gill |
DOI | 10.1016/j.aquatox.2016.06.024 |
Funding Organization | Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China |
Indexed By | SCI |
Language | 英语 |
Funding Project | Students Independent Innovation Program of Wuhan University of Technology[2015-HS-B1-11] ; National High-Tech Research and Development Program of China[2013AA065804] |
WOS Research Area | Marine & Freshwater Biology ; Toxicology |
WOS Subject | Marine & Freshwater Biology ; Toxicology |
WOS ID | WOS:000381529700049 |
WOS Keyword | FRESH-WATER FISH ; TROUT ONCORHYNCHUS-MYKISS ; CHANNA-PUNCTATA BLOCH ; OXIDATIVE STRESS ; CARBONIC-ANHYDRASE ; PSP TOXINS ; RAINBOW-TROUT ; ALEXANDRIUM-FUNDYENSE ; ATPASE ACTIVITIES ; ACUTE EXPOSURE |
Publisher | ELSEVIER SCIENCE BV |
Funding Organization | Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/34665 |
Collection | 藻类生物学及应用研究中心_期刊论文 |
Corresponding Author | Zhang, Delu; Hu, Chunxiang |
Affiliation | 1.Wuhan Univ Technol, Coll Chem Chem Engn & Life Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China 2.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China |
Recommended Citation GB/T 7714 | Zhang, Delu,Liu, Siyi,Zhang, Jing,et al. In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill[J]. AQUATIC TOXICOLOGY,2016,177(1):484-493. |
APA | Zhang, Delu,Liu, Siyi,Zhang, Jing,Zhang, Jian Kong,Hu, Chunxiang,&Liu, Yongding.(2016).In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill.AQUATIC TOXICOLOGY,177(1),484-493. |
MLA | Zhang, Delu,et al."In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill".AQUATIC TOXICOLOGY 177.1(2016):484-493. |
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