IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill
Zhang, Delu1; Liu, Siyi1; Zhang, Jing2; Zhang, Jian Kong1; Hu, Chunxiang3; Liu, Yongding3
2016-08-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume177Issue:1Pages:484-493
Abstract

Aphantoxins, neurotoxins or paralytic shellfish poisons (PSPs) generated by Aphanizomenon flos-aquae, are a threat to environmental safety and human health in eutrophic waters worldwide. The molecular mechanisms of neurotoxin function have been studied; however, the effects of these neurotoxins on oxidative stress, ion transport, gas exchange, and branchial ultrastructure in fish gills are not fully understood. Aphantoxins extracted from A. flos-aquae DC-1 were detected by high-performance liquid chromatography. The major ingredients were gonyautoxins 1 and 5 and neosaxitoxin, which comprised 34.04%, 21.28%, and 12.77% of the total, respectively. Zebrafish (Danio rerio) were administered A. fibs aquae DC-1 aphantoxins at 5.3 or 7.61 mu g saxitoxin equivalents (eq)/kg (low and high doses, respectively) by intraperitoneal injection. The activities of Na+-K+-ATPase (NKA), carbonic anhydrase (CA), and lactate dehydrogenase (LDH), ultrastructural alterations in chloride and epithelial cells, and reactive oxygen species (ROS) and total antioxidative capacity (T-AOC) were investigated in the gills during the first 24 h after exposure. Aphantoxins significantly increased the level of ROS and decreased the T-AOC in zebrafish gills from 3 to 12 h post-exposure, suggesting an induction of oxidative stress and inhibition of antioxidant capacity. Reduced activities of NKA and CA demonstrated abnormal ion transport and gas exchange in the gills of aphantoxin-treated fish. Toxin administration also resulted in increased LDH activity and ultrastructural alterations in chloride and epithelial cells, suggesting a disruption of function and structure in zebrafish gills. The observed abnormalities in zebrafish gills occurred in a time-and dose-dependent manner. These findings demonstrate that aphantoxins or PSPs may inhibit ion transport and gas exchange, increase LDH activity, and result in ultrastructural damage to the gills through elevations in oxidative stress and reduced antioxidant capacity. These effects of aphantoxins in the gills of zebrafish suggest an induction of respiratory toxicity. The parameters investigated in this study may be also considered as biomarkers for studying aphantoxin/PSP exposure and cyanobacterial blooms in nature. (C) 2016 Elsevier B.V. All rights reserved.

KeywordCarbonic anhydrase Cyanobacterial neurotoxin Na+-K+-ATPase Respiratory toxicity Zebrafish gill
DOI10.1016/j.aquatox.2016.06.024
Funding OrganizationStudents Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China
Indexed BySCI
Language英语
Funding ProjectStudents Independent Innovation Program of Wuhan University of Technology[2015-HS-B1-11] ; National High-Tech Research and Development Program of China[2013AA065804]
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000381529700049
WOS KeywordFRESH-WATER FISH ; TROUT ONCORHYNCHUS-MYKISS ; CHANNA-PUNCTATA BLOCH ; OXIDATIVE STRESS ; CARBONIC-ANHYDRASE ; PSP TOXINS ; RAINBOW-TROUT ; ALEXANDRIUM-FUNDYENSE ; ATPASE ACTIVITIES ; ACUTE EXPOSURE
PublisherELSEVIER SCIENCE BV
Funding OrganizationStudents Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; Students Independent Innovation Program of Wuhan University of Technology ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34665
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorZhang, Delu; Hu, Chunxiang
Affiliation1.Wuhan Univ Technol, Coll Chem Chem Engn & Life Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China
2.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Delu,Liu, Siyi,Zhang, Jing,et al. In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill[J]. AQUATIC TOXICOLOGY,2016,177(1):484-493.
APA Zhang, Delu,Liu, Siyi,Zhang, Jing,Zhang, Jian Kong,Hu, Chunxiang,&Liu, Yongding.(2016).In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill.AQUATIC TOXICOLOGY,177(1),484-493.
MLA Zhang, Delu,et al."In vivo effects of Aphanizomenon flos-aquae DC-1 aphantoxins on gas exchange and ion equilibrium in the zebrafish gill".AQUATIC TOXICOLOGY 177.1(2016):484-493.
Files in This Item:
File Name/Size DocType Version Access License
1-s2.0-S0166445X1630(2953KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Delu]'s Articles
[Liu, Siyi]'s Articles
[Zhang, Jing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Delu]'s Articles
[Liu, Siyi]'s Articles
[Zhang, Jing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Delu]'s Articles
[Liu, Siyi]'s Articles
[Zhang, Jing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0166445X16301874-main.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.