Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny. | |
Li, Xiaochuang1,2; Li, Shouchun3; Kong, Renqiu1; Li, Renhui1 | |
2016-07-01 | |
Source Publication | HARMFUL ALGAE
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ISSN | 1568-9883 |
Volume | 57Issue:1Pages:88-97 |
Abstract | The cyanobacterial genera Raphidiopsis and Cylindrospermopsis morphologically resembled each other and are difficult to distinguish, especially when heterocytes do not form in Cylindrospermopsis species. The phylogeny based on multiple gene loci sequences in previous studies cannot divide the strains of these two genera into separate clusters. In the present study, four gene loci 16S rRNA, ITS-L, cpcBA-IGS and rpoC1, sequenced from many Chinese strains (44 Cylindrospermopsis strains and 16 Raphidiopsis strains), were analyzed to infer the phylogenetic relationship between the two genera. According to the 16S rRNA, cpcBA-IGS and rpoC1 gene phylogeny, Cylindrospermopsis and Raphidiopsis strains are intermixed and divide into two or three groups. By contrast, the ITS-L analysis implied that they respectively form two separate and unique clusters. A fragment of 7 bp (RAGAAACT) in ITS-L was conserved for Raphidiopsis strains, which could be distinguished from Cylindrospermopsis strains. A pair of primers targeting the ITS-L fragment specific to Raphidiopsis strains were designed and further verified. (C) 2016 Elsevier B.V. All rights reserved. |
Keyword | Cylindrospermopsis ITS-L Phylogeny Raphidiopsis Taxonomy |
DOI | 10.1016/j.hal.2016.06.003 |
Funding Organization | National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China |
Indexed By | SCI |
Language | 英语 |
Funding Project | National Science Foundation China[NSFC 31170189] ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China[2015FB06] |
WOS Research Area | Marine & Freshwater Biology |
WOS Subject | Marine & Freshwater Biology |
WOS ID | WOS:000381544600009 |
WOS Keyword | POPULATION-DYNAMICS ; SUBBA RAJU ; MEDITERRANEA SKUJA ; FLORIDA LAKES ; 1ST REPORT ; RACIBORSKII ; RESERVOIRS ; DOMINANCE ; SEQUENCES ; RELEASE |
Publisher | ELSEVIER SCIENCE BV |
Funding Organization | National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/34659 |
Collection | 藻类生物学及应用研究中心_期刊论文 |
Corresponding Author | Li, Renhui |
Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Jiangxi Normal Univ, Coll Life Sci, Nanchang 330027, Peoples R China |
Recommended Citation GB/T 7714 | Li, Xiaochuang,Li, Shouchun,Kong, Renqiu,et al. Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.[J]. HARMFUL ALGAE,2016,57(1):88-97. |
APA | Li, Xiaochuang,Li, Shouchun,Kong, Renqiu,&Li, Renhui.(2016).Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny..HARMFUL ALGAE,57(1),88-97. |
MLA | Li, Xiaochuang,et al."Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.".HARMFUL ALGAE 57.1(2016):88-97. |
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