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Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP
Bi, Yanzhen1; Hua, Zaidong1; Liu, Ximei1; Hua, Wenjun1; Ren, Hongyan1; Xiao, Hongwei1; Zhang, Liping1; Li, Li1; Wang, Zhirui2; Laible, Gotz3; Wang, Yan4; Dong, Faming2; Zheng, Xinmin1
2016-08-17
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume6Issue:1Pages:12
Abstract

Predictable, clean genetic modification (GM) in livestock is important for reliable phenotyping and biosafety. Here we reported the generation of isozygous, functional myostatin (MSTN) knockout cloned pigs free of selectable marker gene (SMG) by CRISPR/Cas9 and Cre/LoxP. CRISPR/Cas9-mediated homologous recombination (HR) was exploited to knock out (KO) one allele of MSTN in pig primary cells. Cre recombinase was then used to excise the SMG with an efficiency of 82.7%. The SMG-free non-EGFP cells were isolated by flow cytometery and immediately used as donor nuclei for nuclear transfer. A total of 685 reconstructed embryos were transferred into three surrogates with one delivering two male live piglets. Molecular testing verified the mono-allelic MSTN KO and SMG deletion in these cloned pigs. Western blots showed approximately 50% decrease in MSTN and concurrent increased expression of myogenic genes in muscle. Histological examination revealed the enhanced myofiber quantity but myofiber size remained unaltered. Ultrasonic detection showed the increased longissimus muscle size and decreased backfat thickness. Precision editing of pig MSTN gene has generated isozygous, SMG-free MSTN KO cloned founders, which guaranteed a reliable route for elite livestock production and a strategy to minimize potential biological risks.

DOI10.1038/srep31729
Funding OrganizationChina Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences
Indexed BySCI
Language英语
Funding ProjectChina Major Program of Genetically Modified Organism New Species Cultivation[2016ZX08006001-005] ; China Major Program of Genetically Modified Organism New Species Cultivation[2016ZX08010003-006] ; China Major Program of Genetically Modified Organism New Species Cultivation[2016ZX08006002-006] ; Hubei Key Technology Program[2014BBB010] ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province[2016-620-000-001-027] ; Youth Foundation of Hubei Academy of AgroSciences[2014NKYJJ02] ; Youth Foundation of Hubei Academy of AgroSciences[2016NKYJJ19]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000381480800001
WOS KeywordZINC-FINGER NUCLEASES ; DOUBLE-MUSCLED PHENOTYPE ; EMBRYONIC STEM-CELLS ; MYOSTATIN GENE ; CRE RECOMBINASE ; EFFICIENT GENERATION ; SKELETAL-MUSCLE ; BOVINE GENOME ; CATTLE ; EXPRESSION
PublisherNATURE PUBLISHING GROUP
Funding OrganizationChina Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; China Major Program of Genetically Modified Organism New Species Cultivation ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Hubei Key Technology Program ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Innovation Center for Agricultural Sciences and Technologies of Hubei Province ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences ; Youth Foundation of Hubei Academy of AgroSciences
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Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34616
Collection其他_期刊论文
Corresponding AuthorDong, Faming; Zheng, Xinmin
Affiliation1.Hubei Acad AgroSci, Hubei Inst Anim Sci & Vet Med, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430064, Peoples R China
2.Henan Univ Sci & Technol, Coll Anim Sci, 263 Kaiyuan Ave, Luoyang 471023, Peoples R China
3.AgResearch, Ruakura Res Ctr, Private Bag 3123, Hamilton, New Zealand
4.Chinese Acad Sci, Inst Hydrobiol, Anal & Testing Ctr, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Bi, Yanzhen,Hua, Zaidong,Liu, Ximei,et al. Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP[J]. SCIENTIFIC REPORTS,2016,6(1):12.
APA Bi, Yanzhen.,Hua, Zaidong.,Liu, Ximei.,Hua, Wenjun.,Ren, Hongyan.,...&Zheng, Xinmin.(2016).Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP.SCIENTIFIC REPORTS,6(1),12.
MLA Bi, Yanzhen,et al."Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP".SCIENTIFIC REPORTS 6.1(2016):12.
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