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Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically
Li Yeguang1; Miao Fengping1; Geng Yahong1; Lu Dayan1; Zhang Chengwu2; Zeng Mingtao3
2012
Source PublicationChinese Journal of Oceanology and Limnology
ISSN0254-4059
Volume30Issue:4Pages:627
Abstract

The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematococcus crude extract were investigated, and a spectrophotometric method for precise quantification of the astaxanthin based on the method of Boussiba et al. was established. According to Boussiba's method, alkali treatment destroys chlorophyll. However, we found that: 1) carotenoid content declined for about 25% in Haematococcus fresh cysts and up to 30% in dry powder of Haematococcus broken cysts after alkali treatment; and 2) dimethyl sulfoxide (DMSO)-extracted chlorophyll of green Haematococcus bares little absorption at 520-550 nm. Interestingly, a good linear relationship existed between absorbance at 530 nm and astaxanthin content, while an unknown interference at 540-550 nm was detected in our study. Therefore, with 530 nm as working wavelength, the alkali treatment to destroy chlorophyll was not necessary and the influence of chlorophyll, other carotenoids, and the unknown interference could be avoided. The astaxanthin contents of two samples were measured at 492 nm and 530 nm; the measured values at 530 nm were 2.617 g/100 g and 1.811 g/100 g. When compared with the measured values at 492 nm, the measured values at 530 nm decreased by 6.93% and 11.96%, respectively. The measured values at 530 nm are closer to the true astaxanthin contents in the samples. The data show that 530 nm is the most suitable wave length for spectrophotometric determination to the astaxanthin in Haematococcus crude extract.

Language英语
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34565
Collection藻类生物技术和生物能源研发中心_期刊论文
Affiliation1.中国科学院武汉植物园
2.中国科学院水生生物研究所
3.德克萨斯理工大学
Recommended Citation
GB/T 7714
Li Yeguang,Miao Fengping,Geng Yahong,et al. Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically[J]. Chinese Journal of Oceanology and Limnology,2012,30(4):627.
APA Li Yeguang,Miao Fengping,Geng Yahong,Lu Dayan,Zhang Chengwu,&Zeng Mingtao.(2012).Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically.Chinese Journal of Oceanology and Limnology,30(4),627.
MLA Li Yeguang,et al."Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically".Chinese Journal of Oceanology and Limnology 30.4(2012):627.
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