IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix)
Liu, Haiyang1,2; Pang, Meixia1,2; Yu, Xiaomu1; Zhou, Ying1,2; Tong, Jingou1; Fu, Beide1
2018-06-01
Source PublicationDNA RESEARCH
ISSN1340-2838
Volume25Issue:3Pages:257-264
Abstract

Sex-specific markers are powerful tools for identifying sex-determination system in various animals. Bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix) are two of the most important edible fish in Asia, which have a long juvenility period that can lasts for 4-5 years. In this study, we found one sex-specific marker by next-generation sequencing together with bioinformatics analysis in bighead carp. The male-specific markers were used to perform molecular sexing in the progenies of artificial gynogenetic diploids and found all progenies (n = 160) were females. Meanwhile, around 1 : 1 sex ratio was observed in a total of 579 juvenile offspring from three other families. To further extend the male-specific region, we performed genome walking and got a male-specific sequence of 8,661 bp. Five pairs of primers were designed and could be used to efficiently distinguish males from females in bighead carp and silver carp. The development of these male-specific markers and results of their molecular sexing in different populations provide strong evidence for a sex determination system of female homogametry or male heterogametry (XX/XY) in bighead carp and silver carp. To the best of our knowledge, this is the first report of effective sex-specific markers in these two large carp species.

Keywordsex-specific markers XX/XY sex determination system bighead carp and silver carp
DOI10.1093/dnares/dsx054
Funding OrganizationNSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC
Indexed BySCI
Language英语
Funding ProjectNSFC[31472268] ; NSFC[31502153] ; FEBL[2016FBZ05] ; DAC[2009045]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000439825000003
WOS KeywordYELLOW CATFISH ; CHROMOSOME EVOLUTION ; PROVIDES INSIGHTS ; ARISTICHTHYS-NOBILIS ; ONCORHYNCHUS-MYKISS ; GENOME SEQUENCE ; CYPRINUS-CARPIO ; LINKED MARKERS ; RAINBOW-TROUT ; COMMON CARP
PublisherOXFORD UNIV PRESS
Funding OrganizationNSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; NSFC ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; FEBL ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC ; DAC
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34510
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorFu, Beide
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Liu, Haiyang,Pang, Meixia,Yu, Xiaomu,et al. Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix)[J]. DNA RESEARCH,2018,25(3):257-264.
APA Liu, Haiyang,Pang, Meixia,Yu, Xiaomu,Zhou, Ying,Tong, Jingou,&Fu, Beide.(2018).Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix).DNA RESEARCH,25(3),257-264.
MLA Liu, Haiyang,et al."Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix)".DNA RESEARCH 25.3(2018):257-264.
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