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Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs
Lu,Suxiang1,2; Xiong,Qian3; Du,Kang1; Gan,Xiaoni1; Wang,Xuzhen1; Yang,Liandong1; Wang,Ying1; Ge,Feng3; He,Shunping1
2019-11-20
Source PublicationProteome Science
ISSN1477-5956
Volume17Issue:1Pages:1-10
Abstract

AbstractBackgroundPolypterus senegalus can fully regenerate its pectoral lobed fins, including a complex endoskeleton, with remarkable precision. However, despite the enormous potential of this species for use in medical research, its regeneration mechanisms remain largely unknown.MethodsTo identify the differentially expressed proteins (DEPs) during the early stages of lobed fin regeneration in P. senegalus, we performed a differential proteomic analysis using isobaric tag for relative and absolute quantitation (iTRAQ) approach based quantitative proteome from the pectoral lobed fins at 3 time points. Furthermore, we validated the changes in protein expression with multiple-reaction monitoring (MRM) analysis.ResultsThe experiment yielded a total of 3177 proteins and 15,091 unique peptides including 1006 non-redundant (nr) DEPs. Of these, 592 were upregulated while 349 were downregulated after lobed fin amputation when compared to the original tissue. Bioinformatics analyses showed that the DEPs were mainly associated with Ribosome and RNA transport, metabolic, ECM-receptor interaction, Golgi and endoplasmic reticulum, DNA replication, and Regulation of actin cytoskeleton.ConclusionsTo our knowledge, this is the first proteomic research to investigate alterations in protein levels and affected pathways in bichirs’ lobe-fin/limb regeneration. In addition, our study demonstrated a highly dynamic regulation during lobed fin regeneration in P. senegalus. These results not only provide a comprehensive dataset on differentially expressed proteins during the early stages of lobe-fin/limb regeneration but also advance our understanding of the molecular mechanisms underlying lobe-fin/limb regeneration.

KeywordQuantitative proteome Polypterus senegalus Limb Regrowth Blastema
DOI10.1186/s12953-019-0153-0
Language英语
WOS IDBMC:10.1186/s12953-019-0153-0
PublisherBioMed Central
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34481
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorHe,Shunping
Affiliation1.Key Laboratory of Aquatic Biodiversity and Conservation of the Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei, China
2.Present address: Medical College of Pingdingshan University, Pingdingshan 467000, Henan Province, China.
3.Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei, China.
Recommended Citation
GB/T 7714
Lu,Suxiang,Xiong,Qian,Du,Kang,et al. Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs[J]. Proteome Science,2019,17(1):1-10.
APA Lu,Suxiang.,Xiong,Qian.,Du,Kang.,Gan,Xiaoni.,Wang,Xuzhen.,...&He,Shunping.(2019).Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs.Proteome Science,17(1),1-10.
MLA Lu,Suxiang,et al."Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs".Proteome Science 17.1(2019):1-10.
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