IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Nucleoporin 62-Like Protein Is Required for the Development of Pharyngeal Arches through Regulation of Wnt/beta-Catenin Signaling and Apoptotic Homeostasis in Zebrafish
Yang, Xiaojie; Li, Xixi; Gu, Qilin; Li, Qing; Cui, Zongbin
2019-09-01
Source PublicationCELLS
Volume8Issue:9Pages:17
Abstract

We have previously observed the predominant expression of nucleoporin 62-like (Nup62l) mRNA in the pharyngeal region of zebrafish, which raises the question whether Nup62l has important implications in governing the morphogenesis of pharyngeal arches (PA) in zebrafish. Herein, we explored the functions of Nup62l in PA development. The disruption of Nup62l with a CRISPR/Cas9-dependent gene knockout approach led to defective PA, which was characterized by a thinned and shortened pharyngeal region and a significant loss of pharyngeal cartilages. During pharyngeal cartilage formation, prechondrogenic condensation and chondrogenic differentiation were disrupted in homozygous nup62l-mutants, while the specification and migration of cranial neural crest cells (CNCCs) were unaffected. Mechanistically, the impaired PA region of nup62l-mutants underwent extensive apoptosis, which was mainly dependent on activation of p53-dependent apoptotic pathway. Moreover, aberrant activation of a series of apoptotic pathways in nup62l-mutants is closely associated with the inactivation of Wnt/beta-catenin signaling. Thus, these findings suggest that the regulation of Wnt/beta-catenin activity by Nup62l is crucial for PA formation in zebrafish.

KeywordNup62l craniofacial development pharyngeal arches Wnt beta-catenin signaling apoptosis
DOI10.3390/cells8091038
Funding OrganizationNational Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI
Language英语
Funding ProjectNational Key R & D Program of China[2018YFA0800503] ; National Natural Science Foundation of China[31571504] ; National Natural Science Foundation of China[31871463]
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000489103800097
WOS KeywordNEURAL CREST ; BETA-CATENIN ; NUCLEAR IMPORT ; GENE ; EXPRESSION ; COMPLEX ; JAW ; PROLIFERATION ; ENDOTHELIN-1 ; INACTIVATION
PublisherMDPI
Funding OrganizationNational Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Key R & D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34468
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorCui, Zongbin
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Yang, Xiaojie,Li, Xixi,Gu, Qilin,et al. Nucleoporin 62-Like Protein Is Required for the Development of Pharyngeal Arches through Regulation of Wnt/beta-Catenin Signaling and Apoptotic Homeostasis in Zebrafish[J]. CELLS,2019,8(9):17.
APA Yang, Xiaojie,Li, Xixi,Gu, Qilin,Li, Qing,&Cui, Zongbin.(2019).Nucleoporin 62-Like Protein Is Required for the Development of Pharyngeal Arches through Regulation of Wnt/beta-Catenin Signaling and Apoptotic Homeostasis in Zebrafish.CELLS,8(9),17.
MLA Yang, Xiaojie,et al."Nucleoporin 62-Like Protein Is Required for the Development of Pharyngeal Arches through Regulation of Wnt/beta-Catenin Signaling and Apoptotic Homeostasis in Zebrafish".CELLS 8.9(2019):17.
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