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patD, a Gene Regulated by NtcA, Is Involved in the Optimization of Heterocyst Frequency in the Cyanobacterium Anabaena sp. Strain PCC 7120
Wang, Li1; Lin, Gui-Ming1,2; Niu, Tian-Cai1,2; Zhang, Shao-Ran1; Zhang, Ju-Yuan1; Tang, Guo-Fang1; Chen, Wenli1; Zhang, Cheng-Cai2,3
Corresponding AuthorChen, Wenli(wlchen@mail.hzau.edu.cn) ; Zhang, Cheng-Cai(cczhang@ihb.ac.cn)
2019-10-01
Source PublicationJOURNAL OF BACTERIOLOGY
ISSN0021-9193
Volume201Issue:21Pages:13
AbstractIn the filamentous multicellular cyanobacterium Anabaena sp. strain PCC 7120, 5 to 10% of the cells differentiate into heterocysts, which are specialized in N-2 fixation. Heterocysts and vegetative cells are mutually dependent for filament growth through nutrient exchange. Thus, the heterocyst frequency should be optimized to maintain the cellular carbon and nitrogen (C/N) balance for filament fitness in the environment. Here, we report the identification of patD, whose expression is directly activated in developing cells by the transcription factor NtcA. The inactivation of patD increases heterocyst frequency and promotes the upregulation of the positive regulator of heterocyst development hetR, whereas its overexpression decreases the heterocyst frequency. The change in heterocyst frequency resulting from the inactivation of patD leads to the reduction in competitiveness of the filaments under combined-nitrogen-depleted conditions. These results indicate that patD regulates heterocyst frequency in Anabaena sp. PCC 7120, ensuring its optimal filament growth. IMPORTANCE Microorganisms have evolved various strategies in order to adapt to the environment and compete with other organisms. Heterocyst differentiation is a prokaryotic model for studying complex cellular regulation. The NtcA-regulated gene patD controls the ratio of heterocysts relative to vegetative cells on the filaments of Anabaena sp. strain PCC 7120. Such a regulation provides a mechanism through which carbon fixation by vegetative cells and nitrogen fixation by heterocysts are properly balanced to ensure optimal growth and keep a competitive edge for long-term survival.
Keywordcyanobacteria fitness control heterocyst frequency nitrogen fixation
DOI10.1128/JB.00457-19
Funding OrganizationFundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectFundamental Research Funds for the Central Universities[2014PY055] ; Chinese Academy of Sciences (Frontier Research Program)[QYZDJ-SSW-SMC016] ; Institute of Hydrobiology ; National Science Foundation of China[31170048] ; National Science Foundation of China[31570048]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000489038600010
WOS KeywordPATTERN-FORMATION ; CELLULAR-DIFFERENTIATION ; NITROGEN ASSIMILATION ; TRANSCRIPTION FACTOR ; ESCHERICHIA-COLI ; HETR ; EXPRESSION ; PROTEIN ; PCC-7120 ; GROWTH
PublisherAMER SOC MICROBIOLOGY
Funding OrganizationFundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Chinese Academy of Sciences (Frontier Research Program) ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; Institute of Hydrobiology ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34440
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorChen, Wenli; Zhang, Cheng-Cai
Affiliation1.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
3.Aix Marseille Univ & Wuhan Univ Technol, Inst WUT AMU, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wang, Li,Lin, Gui-Ming,Niu, Tian-Cai,et al. patD, a Gene Regulated by NtcA, Is Involved in the Optimization of Heterocyst Frequency in the Cyanobacterium Anabaena sp. Strain PCC 7120[J]. JOURNAL OF BACTERIOLOGY,2019,201(21):13.
APA Wang, Li.,Lin, Gui-Ming.,Niu, Tian-Cai.,Zhang, Shao-Ran.,Zhang, Ju-Yuan.,...&Zhang, Cheng-Cai.(2019).patD, a Gene Regulated by NtcA, Is Involved in the Optimization of Heterocyst Frequency in the Cyanobacterium Anabaena sp. Strain PCC 7120.JOURNAL OF BACTERIOLOGY,201(21),13.
MLA Wang, Li,et al."patD, a Gene Regulated by NtcA, Is Involved in the Optimization of Heterocyst Frequency in the Cyanobacterium Anabaena sp. Strain PCC 7120".JOURNAL OF BACTERIOLOGY 201.21(2019):13.
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