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PfHMGB2 protects yellow catfish (Pelteobagrus fulvidraco) from bacterial infection by promoting phagocytosis and proliferation of PBL
Wang, Yun1,2; Yang, Yanyan2; Chen, Qianying2; Zhai, Hanfei2; Xie, Zhaohui2; Ke, Fei3
Corresponding AuthorWang, Yun(y-wang401@163.com)
2019-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume93Pages:567-574
AbstractHMGB2, a member of the high mobility group box family, plays an important role in host immune responses. However, the mechanism of action of HMGB2 is not well understood. Herein, a homologue from yellow catfish (Pelteobagrus fulvidraco) was cloned and named PfHMGB2. The deduced amino acid sequence of PfHMGB2 possessed a typical tripartite structure (two DNA binding boxes and an acid tail) and shared 90% identity with the predicted HMGB2 from I. punctatus. The mRNA of PfHMGB2 was widely distributed in all 11 tested tissues in healthy fish bodies and was significantly induced in the liver and head kidney when yellow catfish were injected with inactivated Aeromonas hydrophila. Consistently, PfHMGB2 mRNA could also be induced in yellow catfish peripheral blood leucocytes (PBL) by lipopolysaccharide. The recombinant PfHMGB2 protein was purified from E. coli BL21 (DE3):pET-28a/PfHMGB2 and showed DNA-binding affinity. Moreover, rPfHMGB2 improved the phagocytosis and proliferation activity and upregulated the mRNA expression of the pro-inflammatory cytokine TNFa in yellow catfish PBL. These results indicated that PfHMGB2 could protect yellow catfish from pathogen infection by activating PBL.
KeywordHMGB2 DNA affinity Antibacterial activity Phagocytosis Proliferation TNF alpha Yellow catfish
DOI10.1016/j.fsi.2019.08.007
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31702388]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000488998300063
WOS KeywordGROUP BOX 1 ; MOBILITY GROUP B2 ; HMGB PROTEINS ; DNA-BINDING ; CYSTEINE RESIDUES ; GENE ; CLONING ; MICE ; IDENTIFICATION ; MOLECULE
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34377
Collection其他_期刊论文
Corresponding AuthorWang, Yun
Affiliation1.Jianghan Univ, Inst Environm & Hlth, Hubei Key Lab Environm & Hlth Effects Persistent, Wuhan 430056, Hubei, Peoples R China
2.Henan Univ Urban Construct, Henan Prov Key Lab Water Pollut Control & Rehabil, Pingdingshan 467036, Henan, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yun,Yang, Yanyan,Chen, Qianying,et al. PfHMGB2 protects yellow catfish (Pelteobagrus fulvidraco) from bacterial infection by promoting phagocytosis and proliferation of PBL[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:567-574.
APA Wang, Yun,Yang, Yanyan,Chen, Qianying,Zhai, Hanfei,Xie, Zhaohui,&Ke, Fei.(2019).PfHMGB2 protects yellow catfish (Pelteobagrus fulvidraco) from bacterial infection by promoting phagocytosis and proliferation of PBL.FISH & SHELLFISH IMMUNOLOGY,93,567-574.
MLA Wang, Yun,et al."PfHMGB2 protects yellow catfish (Pelteobagrus fulvidraco) from bacterial infection by promoting phagocytosis and proliferation of PBL".FISH & SHELLFISH IMMUNOLOGY 93(2019):567-574.
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