IHB OpenIR  > 专利
一种柱状黄杆菌基因定向敲除质粒及应用
聂品; 李楠; 秦婷; 张晓林
Rights Holder中国科学院水生生物研究所
Date Available2015-10-07
Country中国
Subtype发明专利
Abstract本发明公开了一种柱状黄杆菌基因定向敲除质粒及应用,本发明提供了一种柱状黄杆菌基因定向敲除质粒,其序列为SEQ ID NO.1所示。利用该质粒,可以实现对柱状黄杆菌目的DNA片段的定向敲除、对其开展功能研究,进而实现对柱状黄杆菌致病机理的研究,可用于构建柱状黄杆菌基因缺失株或构建弱毒疫苗,本发明构建的定向敲除质粒,能够使sacB基因在黄杆菌中很好地表达,赋予其蔗糖敏感性,获得了无标记基因的突变株,成功克服了柱状黄杆菌基因定向敲除中遇到的最大障碍。
Application Date2015-07-08
Language中文
Application NumberCN201510398413.4
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/34361
Collection专利
Recommended Citation
GB/T 7714
聂品,李楠,秦婷,等. 一种柱状黄杆菌基因定向敲除质粒及应用.
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