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一种利用荧光检测微囊藻细胞DNA损伤的方法
吴振斌; 鲁志营; 刘碧云; 何燕; 贺锋; 周巧红; 徐栋; 张甬元
2016-05-04
Rights Holder中国科学院水生生物研究所
Date Available2016-05-04
Country中国
Subtype发明专利
Abstract

本发明公开一种利用荧光检测微囊藻细胞DNA损伤的方法,其步骤:(a)将待检测样品和对照样品均分别平均分成三小组;(b)去除细胞角质鞘:离心收集样品中藻细胞并重悬浮于SE缓冲液中洗涤;(c)细胞裂解:将细胞分别重悬浮于Lysis裂解液,加入蛋白酶K和十二烷基磺酸钠,使细胞裂解;(d)细胞DNA链解旋:改变pH,使T、P和B样品在不同条件下解旋;(e)染色:分别向以上T、P和B样品中加入Hoechest 33258染色;(f)荧光测定:离心后于荧光检测器中检测上清液的荧光强度;(g)结果计算:根据待测样品和对照样品中T、B和P样品的荧光来计算DNA链的断裂水平。本方法易于掌握,并且灵敏度高,DNA链上单个断裂位点即可检测到。

Application Date2016-02-26
Language中文
Application NumberCN105543399A
Document Type专利
Identifierhttp://ir.ihb.ac.cn/handle/342005/34331
Collection专利
Recommended Citation
GB/T 7714
吴振斌,鲁志营,刘碧云,等. 一种利用荧光检测微囊藻细胞DNA损伤的方法[P]. 2016-05-04.
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