IHB OpenIR  > 藻类生物技术和生物能源研发中心  > 期刊论文
A New Paradigm for Producing Astaxanthin From the Unicellular Green Alga Haematococcus pluvialis
Zhang, Zhen1,2; Wang, Baobei2,4; Hu, Qiang3; Sommerfeld, Milton2; Li, Yuanguang1; Han, Danxiang3
2016-10-01
Source PublicationBIOTECHNOLOGY AND BIOENGINEERING
ISSN0006-3592
Volume113Issue:10Pages:2088-2099
Abstract

The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and cell deterioration or death under stress conditions (e.g., high light, nitrogen starvation) has limited the astaxanthin production. In this study, a new paradigm that integrated heterotrophic cultivation, acclimation of heterotrophically grown cells to specific light/nutrient regimes, followed by induction of astaxanthin accumulation under photoautotrophic conditions was developed. First, the environmental conditions such as pH, carbon source, nitrogen regime, and light intensity, were optimized to induce astaxanthin accumulation in the dark-grown cells. Althoughmoderate astaxanthin content (e.g., 1% of dry weight) and astaxanthin productivity (2.5mg L-1 day(-1)) were obtained under the optimized conditions, a considerable number of cells died off when subjected to stress for astaxanthin induction. To minimize the susceptibility of dark-grown cells to light stress, the algal cells were acclimated, prior to light induction of astaxanthin biosynthesis, under moderate illumination in the presence of nitrogen. Introduction of this strategy significantly reduced the cell mortality rate under high-light and resulted in increased cellular astaxanthin content and astaxanthin productivity. The productivity of astaxanthin was further improved to 10.5mg L-1 day(-1) by implementation of such a strategy in a bubbling column photobioreactor. Biochemical and physiological analyses suggested that rebuilding of photosynthetic apparatus including D1 protein and PsbO, and recovery of PSII activities, are essential for acclimation of dark-grown cells under photo-induction conditions. (C) 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

Keywordastaxanthin Haematococcus pluvialis heterotrophy acclimation
DOI10.1002/bit.25976
Funding OrganizationState Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province
Indexed BySCI
Language英语
Funding ProjectState Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province[2015CFA022]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000386753600002
WOS KeywordDILUTION-PHOTOINDUCTION CULTIVATION ; DEPENDENT CHLOROPHYLL BIOSYNTHESIS ; CHLAMYDOMONAS-REINHARDTII ; HETEROTROPHIC GROWTH ; FLUORESCENCE ; LACUSTRIS ; CULTURES ; IDENTIFICATION ; CHLOROPLASTS ; MICROALGAE
PublisherWILEY-BLACKWELL
Funding OrganizationState Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province ; National Science Foundation of Hubei Province
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/34170
Collection藻类生物技术和生物能源研发中心_期刊论文
Corresponding AuthorLi, Yuanguang; Han, Danxiang
Affiliation1.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China
2.Arizona State Univ, Dept Human Syst & Environm, Mesa, AZ USA
3.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Hubei, Peoples R China
4.Xiamen Univ, Dept Chem & Biochem Engn, Xiamen, Fujian, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Zhen,Wang, Baobei,Hu, Qiang,et al. A New Paradigm for Producing Astaxanthin From the Unicellular Green Alga Haematococcus pluvialis[J]. BIOTECHNOLOGY AND BIOENGINEERING,2016,113(10):2088-2099.
APA Zhang, Zhen,Wang, Baobei,Hu, Qiang,Sommerfeld, Milton,Li, Yuanguang,&Han, Danxiang.(2016).A New Paradigm for Producing Astaxanthin From the Unicellular Green Alga Haematococcus pluvialis.BIOTECHNOLOGY AND BIOENGINEERING,113(10),2088-2099.
MLA Zhang, Zhen,et al."A New Paradigm for Producing Astaxanthin From the Unicellular Green Alga Haematococcus pluvialis".BIOTECHNOLOGY AND BIOENGINEERING 113.10(2016):2088-2099.
Files in This Item: Download All
File Name/Size DocType Version Access License
bit.25976.pdf(1501KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Zhen]'s Articles
[Wang, Baobei]'s Articles
[Hu, Qiang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Zhen]'s Articles
[Wang, Baobei]'s Articles
[Hu, Qiang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Zhen]'s Articles
[Wang, Baobei]'s Articles
[Hu, Qiang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: bit.25976.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.