| Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver | |
| Tuo, Xun1,2,3; Chen, Jun1; Zhao, Sujuan1,3; Xie, Ping1 | |
| Corresponding Author | Xie, Ping(xieping@ihb.ac.cn) |
| 2016-10-01 | |
| Source Publication | ENVIRONMENTAL TOXICOLOGY
 |
| ISSN | 1520-4081 |
| Volume | 31Issue:10Pages:1206-1216 |
| Abstract | Microcystins (MCs) are common toxins produced by freshwater cyanobacteria, and they represent a potential health risk to aquatic organisms and animals, including humans. Specific inhibition of protein phosphatases 1 and 2A is considered the typical mechanism of MCs toxicity, but the exact mechanism has not been fully elucidated. To further our understanding of the toxicological mechanisms induced by MCs, this study is the first to use a chemical proteomic approach to screen proteins that exhibit special interactions with MC-arginine-arginine (MC-RR) from zebrafish (Danio rerio) liver. Seventeen proteins were identified via affinity blocking test. Integration of the results of previous studies and this study revealed that these proteins play a crucial role in various toxic phenomena of liver induced by MCs, such as the disruption of cytoskeleton assembly, oxidative stress, and metabolic disorder. Moreover, in addition to inhibition of protein phosphate activity, the overall toxicity of MCs was simultaneously modulated by the distribution of MCs in cells and their interactions with other target proteins. These results provide new insight into the mechanisms of hepatotoxicity induced by MCs. (c) 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1206-1216, 2016. |
| Keyword | microcystin-RR chemical proteomics toxicological mechanisms zebrafish liver |
| DOI | 10.1002/tox.22128 |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31322013] ; National Natural Science Foundation of China[31070457] |
| WOS Research Area | Environmental Sciences & Ecology ; Toxicology ; Water Resources |
| WOS Subject | Environmental Sciences ; Toxicology ; Water Resources |
| WOS ID | WOS:000383611900005 |
| WOS Keyword | TANDEM MASS-SPECTROMETRY ; AFFINITY-CHROMATOGRAPHY ; RAT HEPATOCYTES ; INTERMEDIATE-FILAMENTS ; PROTEIN PHOSPHATASES ; PROTEOLYTIC-ENZYMES ; CELLULAR-RESPONSES ; SERINE PROTEASES ; MOUSE-LIVER ; FISH LIVER |
| Publisher | WILEY-BLACKWELL |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/34138 |
| Collection | 水环境工程研究中心_期刊论文 |
| Corresponding Author | Xie, Ping |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Donghu South Rd 7, Wuhan 430072, Peoples R China 2.Nanchang Univ, Basic Chem Expt Ctr, Nanchang 330031, Jiangxi, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Tuo, Xun,Chen, Jun,Zhao, Sujuan,et al. Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver[J]. ENVIRONMENTAL TOXICOLOGY,2016,31(10):1206-1216. |
| APA | Tuo, Xun,Chen, Jun,Zhao, Sujuan,&Xie, Ping.(2016).Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver.ENVIRONMENTAL TOXICOLOGY,31(10),1206-1216. |
| MLA | Tuo, Xun,et al."Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver".ENVIRONMENTAL TOXICOLOGY 31.10(2016):1206-1216. |
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